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Odontogenesis pp 549–562Cite as

Saliva and Gingival Crevicular Fluid (GCF) Collection for Biomarker Screening

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1922))

Abstract

Assaying different biological markers (biomarkers) is commonly used to monitor health status and aid in the diagnosis of diseases. With the recent advances in highly sensitive protein assays, whole saliva (WS) and gingival crevicular fluid (GCF) appear to be fluids that may contain important biomarkers with various applications in dentistry and medicine. Herein, we describe the process of GCF and WS sample collection and preparation for assaying clinically relevant biomarkers in clinical screening trials. Analysis of biomarkers in WS and GCF represents an easy and practical approach for the diagnosis and screening of different pathological conditions particularly in epidemiological surveys.

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Author information

Authors and Affiliations

  1. Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada

    Raed Said

  2. College of Dentistry, University of Saskatchewan, Saskatoon, SK, Canada

    Petros Papagerakis & Raed Said

  3. Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Doohak Kim, Amber A. Ehlert & Kevin K. M. Chung

  4. Department of Surgery, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada

    Silvana Papagerakis

  5. Department of Otolaryngology—Head and Neck Surgery, University of Michigan, Ann Arbor, MI, USA

    Silvana Papagerakis

  6. Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Li Zheng

  7. Department of Orthodontics, The Ohio State University College of Dentistry, Columbus, OH, USA

    Li Zheng

  8. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

Authors
  1. Petros Papagerakis
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  2. Li Zheng
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  3. Doohak Kim
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  4. Raed Said
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  5. Amber A. Ehlert
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  6. Kevin K. M. Chung
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  7. Silvana Papagerakis
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Corresponding authors

Correspondence to Petros Papagerakis or Silvana Papagerakis .

Editor information

Editors and Affiliations

  1. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

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Papagerakis, P. et al. (2019). Saliva and Gingival Crevicular Fluid (GCF) Collection for Biomarker Screening. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_41

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  • DOI: https://doi.org/10.1007/978-1-4939-9012-2_41

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9011-5

  • Online ISBN: 978-1-4939-9012-2

  • eBook Packages: Springer Protocols

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