Abstract
Epidemiological investigations of early childhood oral health rely upon the collection of high-quality clinical measures of health and disease. However, ascertainment of valid and accurate clinical measures presents unique challenges among young, preschool-age children. The paper presents a clinical research protocol for the conduct of oral epidemiological examinations among children, implemented in ZOE 2.0, a large-scale population-based genetic epidemiologic study of early childhood caries (ECC). The protocol has been developed for the collection of information on tooth surface-level dental caries experience and tooth-level developmental defects of the enamel in the primary dentition. Dental caries experience is recorded using visual criteria modified from the International Caries Detection and Assessment System (ICDAS), and measurement of developmental defects is based upon the modified Clarkson and O’Mullane Developmental Defects of the Enamel Index. After a dental prophylaxis (toothbrushing among all children and flossing as needed), children’s teeth are examined by trained and calibrated examiners in community locations, using portable dental equipment, compressed air, and uniform artificial light and magnification conditions. Data are entered directly onto a computer using a custom Microsoft Access-based data entry application. The ZOE 2.0 clinical protocol has been implemented successfully for the conduct of over 6000 research examinations to date, contributing phenotype data to downstream genomics and other “omics” studies of ECC and DDE, as well as traditional clinical and epidemiologic dental research.
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References
Casamassimo PS, Lee JY, Marazita ML, Milgrom P, Chi DL, Divaris K (2014) Improving children’s oral health: an interdisciplinary research framework. J Dent Res 93(10):938–942
Drury TF, Horowitz AM, Ismail AI, Maertens MP, Rozier RG, Selwitz RH (1999 Summer) Diagnosing and reporting early childhood caries for research purposes. A report of a workshop sponsored by the National Institute of Dental and Craniofacial Research, the Health Resources and Services Administration, and the Health Care Financing Administration. J Public Health Dent 59(3):192–197
Divaris K (2016) Predicting dental caries outcomes in children: a “risky” concept. J Dent Res 95(3):248–254
Ballantine J, Carlson JC, Zandona A, Agler CS, Zeldin LP, Rozier G, Roberts MW, Basta PV, Luo J, Antonio-Obese ME, McNeil D, Weyant R, Crout RJ, Slayton R, Levy S, Shaffer JR, Marazita ML, North KE, Divaris K (2018) Exploring the genomic basis of early childhood caries: a pilot study. Int J Paediatr Dent 28(2):217–225
Seow WK (2014) Developmental defects of enamel and dentine: challenges for basic science research and clinical management. Aust Dent J 59(Suppl 1):143–154
Caufield PW, Li Y, Bromage TG (2012 Jun) Hypoplasia-associated severe early childhood caries—a proposed definition. J Dent Res 91(6):544–550
Vieira AR, Modesto A, Marazita M (2014) Caries: review of human genetic research. Caries Res 48:491–506
Shaffer JR, Wang X, Feingold E et al (2011) Genome-wide association scan for childhood caries implicates novel genes. J Dent Res 90(12):1457–1462
Divaris K (2017) Precision dentistry in early childhood: the central role of genomics. Dent Clin North Am 61(3):619–625
Pitts N (2004) “ICDAS”—an international system for caries detection and assessment being developed to facilitate caries epidemiology, research and appropriate clinical management. Community Dent Health 21(3):193–198
Ismail AI, Sohn W, Tellez M, Amaya A, Sen A, Hasson H, Pitts NB (2007) The International Caries Detection and Assessment System (ICDAS): an integrated system for measuring dental caries. Community Dent Oral Epidemiol 35(3):170–178
Banting DW, Amaechi BT, Bader JD, Blanchard P, Gilbert GH, Gullion CM, Holland JC, Makhija SK, Papas A, Ritter AV, Singh ML, Vollmer WM (2011) Examiner training and reliability in two randomized clinical trials of adult dental caries. J Public Health Dent 71(4):335–344
Clarkson J, O’Mullane D (1989) A modified DDE Index for use in epidemiological studies of enamel defects. J Dent Res 68(3):445–450
Ellis RG, Davey KW (1970) The classification and treatment of injuries to the teeth of children: a reference manual for the dental student and the general practitioner. Year Book Medical Publishers, 5th Edition, Chicago
Agler AS, Shungin D, Ferreira Zandoná AG, Basta PV, Luo J, Cantrell J, Pahel Jr. TD, Meyer BD, Shaffer JR, Schäefer AS, North KE, Divaris K (this volume) Protocols, methods and tools for genome-wide association studies (GWAS) of dental traits. Methods Mol Biol 1922:493–509
Shungin D, Basta PV, Azcarate-Peril AM, Ferreira Zandoná AG, Cho H, Wu D, Ginnis J, Cantrell J, Pahel Jr. TD, Roach J, Divaris K (this volume) Protocol for clinical, laboratory and bioinformatics pipelines for oral microbiome studies of dental caries involving metagenomics, metatrascriptomics and metabolomics. Methods Mol Biol 1922:525–548
Frankl SN, Shiere FR, Fogels HR (1962) Should the parent remain with the child in the dental operatory? J Dent Child 29:150–163
Acknowledgment
This work was supported by a grant from the National Institutes of Health, National Institute of Dental and Craniofacial Research, U01-DE025046.
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Ginnis, J. et al. (2019). Measurement of Early Childhood Oral Health for Research Purposes: Dental Caries Experience and Developmental Defects of the Enamel in the Primary Dentition. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_39
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DOI: https://doi.org/10.1007/978-1-4939-9012-2_39
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