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Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues

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Odontogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1922))

Abstract

This chapter describes methods related to the diagnosis of genetic dental diseases. Based on the present knowledge, clinical phenotyping and next-generation sequencing techniques are discussed. Methods necessary for Sanger sequencing, multiplex ligation-dependent probe amplification, and epigenetic modification methods are detailed. In addition, protocols for cell culture establishment and characterization from patients with inherited dental anomalies are described.

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Author information

Authors and Affiliations

  1. Oral Histopathology Laboratory, Department of Dentistry, Health Sciences Faculty, University of Brasília (UnB), Brasília, Brazil

    Bruna Rabelo Amorim, Pollyanna Almeida Costa dos Santos, Caroline Lourenço de Lima & Ana Carolina Acevedo

  2. Molecular Pharmacology Laboratory, Department of Pharmaceutics Science, Health Sciences Faculty, University of Brasília (UnB), Brasília, Brazil

    Caroline Lourenço de Lima

  3. Department of Epigenetics, Dental Research Division, Health Science Institute, Paulista University (UNIP), São Paulo, SP, Brazil

    Denise Carleto Andia

  4. Laboratory of Medical Genetics, Faculty of Medicine, University of Brasília (UnB), Brasília, Brazil

    Juliana Forte Mazzeu

  5. Universidade Estadual de Ciências da Saúde de Alagoas, Maceio, Alagoas, Brazil

    Pollyanna Almeida Costa dos Santos

Authors
  1. Bruna Rabelo Amorim
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  2. Pollyanna Almeida Costa dos Santos
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  3. Caroline Lourenço de Lima
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  4. Denise Carleto Andia
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  5. Juliana Forte Mazzeu
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  6. Ana Carolina Acevedo
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Editor information

Editors and Affiliations

  1. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

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Amorim, B.R., dos Santos, P.A.C., de Lima, C.L., Andia, D.C., Mazzeu, J.F., Acevedo, A.C. (2019). Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_37

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  • DOI: https://doi.org/10.1007/978-1-4939-9012-2_37

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9011-5

  • Online ISBN: 978-1-4939-9012-2

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