Abstract
The earliest steps of animal development depend upon posttranscriptional events that drive the embryonic cell cycle and guide cell fate decisions. The analysis of post-transcriptional regulatory events has relied upon the use of chimeric reporter mRNAs that encode firefly luciferase fused to potential regulatory sequences. A new and more sensitive luciferase developed recently called NanoLuc has the potential to improve reporter studies and provide new insights into the regulation of embryonic processes. Here I describe how to create and analyze reporter mRNAs encoding NanoLuc luciferase using extracts from microinjected Xenopus embryos.
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References
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Acknowledgments
Work in the Sheets lab was supported by NIH grants (R21HD076828, R01HD091921).
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Sheets, M.D. (2019). Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos. In: Pelegri, F. (eds) Vertebrate Embryogenesis. Methods in Molecular Biology, vol 1920. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9009-2_3
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DOI: https://doi.org/10.1007/978-1-4939-9009-2_3
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