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Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons

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Neural Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1919))

Abstract

Dopaminergic (DA) neurons are involved in many critical functions within the central nervous system (CNS), and dopamine neurotransmission impairment underlies a wide range of disorders from motor control deficiencies, such as Parkinson’s disease (PD), to psychiatric disorders, such as alcoholism, drug addictions, bipolar disorders, schizophrenia and depression. Neural stem cell-based technology has potential to play an important role in developing efficacious biological and small molecule therapeutic products for disorders with dopamine dysregulation. Various methods of differentiating DA neurons from pluripotent stem cells have been reported. In this chapter, we describe a simple technique using dopamine-inducing factors (DIFs) to differentiate neural stem cells (NSCs), isolated from induced pluripotent stem cells (iPSCs) into DA neurons.

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Acknowledgment

This work was supported by NeoNeuron LLC.

Disclosures: Dr. Marcel M. Daadi is founder of the biotech company NeoNeuron.

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Authors and Affiliations

  1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA

    Marcel M. Daadi

  2. Department of Radiology, Research Imaging Institute, Cell Systems and Anatomy, Long School of Medicine, University of Texas Health Science Center, San Antonio, TX, USA

    Marcel M. Daadi

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  1. Marcel M. Daadi
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Correspondence to Marcel M. Daadi .

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Editors and Affiliations

  1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA

    Marcel M. Daadi

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Daadi, M.M. (2019). Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons. In: Daadi, M. (eds) Neural Stem Cells. Methods in Molecular Biology, vol 1919. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9007-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9007-8_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9005-4

  • Online ISBN: 978-1-4939-9007-8

  • eBook Packages: Springer Protocols

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