Abstract
When exposed to environmental stresses, bacteria can modulate its fatty acid composition of membrane phospholipids in order to optimize membrane fluidity. Characterization of bacterial membrane fatty acid profiles is thus an interesting indicator of cellular physiological state. The methodology described here aims to improve the recovering of biofilm cells for the characterization of their fatty acid profiles. The saponification reagent is directly applied on the whole biofilm before the removal of cells from the inert surface. In this way, maximum of the cells and their fatty acids can be recovered from the deepest layers of the biofilm.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Costerton JW, Cheng K-J, Geesey GG, Ladd TI, Nickel JC, Dasgupta M, Marrie TJ (1987) Bacterial biofilms in nature and disease. Annu Rev Microbiol 41:435–464
Bridier A, Briandet R, Thomas V, Dubois-Brissonnet F (2011) Resistance of bacterial biofilms to disinfectants: a review. Biofouling 27(9):1017–1032
Denich TJ, Beaudette LA, Lee H, Trevors JT (2003) Effect of selected environmental and physico-chemical factors on bacterial cytoplasmic membranes. J Microbiol Methods 52(2):149–182. https://doi.org/10.1016/s0167-7012(02)00155-0
Parsons JB, Rock C (2013) Bacterial lipids: metabolism and membrane homeostasis. Prog Lipid Res 52(3):249–276. https://doi.org/10.1016/j.plipres.2013.02.002
Dubois-Brissonnet F, Trotier E, Briandet R (2016) The biofilm lifestyle involves an increase in bacterial membrane saturated fatty acids. Front Microbiol 7(1673). https://doi.org/10.3389/fmicb.2016.01673
Miller L, Berger T (1985) Bacteria identification by gas chromatography of whole cell fatty acids. Application note 228–41 Hewlett Packard
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Dubois-Brissonnet, F. (2019). Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells. In: Bridier, A. (eds) Foodborne Bacterial Pathogens. Methods in Molecular Biology, vol 1918. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9000-9_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9000-9_13
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-8999-7
Online ISBN: 978-1-4939-9000-9
eBook Packages: Springer Protocols