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Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

This chapter describes the analysis of signaling pathways in bone cells by the use of western blotting and immunoprecipitation, including a step-by-step guide to cell culture techniques, cellular and subcellular fractionation, protein isolation, purification, measurement, electrophoretic transfer, and detection.

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Author information

Authors and Affiliations

  1. Division Hematology Oncology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA

    Silvia Marino

  2. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

Authors
  1. Silvia Marino
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  2. Aymen I. Idris
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Corresponding author

Correspondence to Aymen I. Idris .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

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Marino, S., Idris, A.I. (2019). Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

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