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Tracking Cancer Cells Colonization in Rodent Bone Using Two-Photon Microscopy

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

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Abstract

Two-photon microscopy has been widely accepted as a powerful tool to provide both qualitative and quantitative information in bone research. This chapter will describe a step-by-step protocol for using two-photon microscopy to track the colonization of cancer cells to bone using frozen bone samples of xenograft mouse models.

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Author information

Authors and Affiliations

  1. Department of Oncology and Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield, Sheffield, UK

    Gloria Allocca & Ning Wang

Authors
  1. Gloria Allocca
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  2. Ning Wang
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Corresponding author

Correspondence to Ning Wang .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

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Allocca, G., Wang, N. (2019). Tracking Cancer Cells Colonization in Rodent Bone Using Two-Photon Microscopy. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_33

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  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_33

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

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