Skip to main content
SpringerLink
Log in
Book cover

Bone Research Protocols pp 507–531Cite as

Analysis of Bone Architecture in Rodents Using Micro-Computed Tomography

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

This chapter describes the use of micro-computed tomography scanning for analyzing bone structure, focussing on rodent bone. It discusses sample preparation, the correct setup of the scanner, the impact of some of the important scanner settings and new applications.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Bouxsein ML, Boyd SK, Christiansen BA, Guldberg RE, Jepsen KJ, Müller R (2010) Guidelines for assessment of bone microstructure in rodents using micro-computed tomography. J Bone Miner Res 25(7):1468–1486

    Article  Google Scholar 

  2. Daroszewska A, Van’t Hof R, Rose L, Rose K, Ralston S (2009) The P392L mutation of SQSTM1 causes a Paget’s disease-like phenotype in mice. J Bone Miner Res 24(Suppl 1):S38

    Google Scholar 

  3. Oliviero S, Lu Y, Viceconti M, Dall’Ara E (2017) Effect of integration time on morphometric, densitometric and mechanical properties of the mouse tibia. J Biomech 65:203–211

    Article  CAS  Google Scholar 

  4. Campbell GM, Sophocleous A (2014) Quantitative analysis of bone and soft tissue by micro-computed tomography: applications to ex vivo and in vivo studies. Bonekey Rep 3:564

    Article  Google Scholar 

  5. Lu Y, Boudiffa M, Dall’Ara E, Bellantuono I, Viceconti M (2015) Development of a protocol to quantify local bone adaptation over space and time: quantification of reproducibility. J Biomech 49(10):2095–2099

    Article  Google Scholar 

  6. Dall’Ara E, Barber D, Viceconti M (2014) About the inevitable compromise between spatial resolution and accuracy of strain measurements for bone tissue: a 3D zero-strain study. J Biomech 47(12):2956–2963

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UK

    Robert J. van ’t Hof

  2. Department of Oncology and Metabolism and Insigneo Institute for in Silico Medicine, University of Sheffield, Sheffield, UK

    Enrico Dall’Ara

Authors
  1. Robert J. van ’t Hof
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Enrico Dall’Ara
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Robert J. van ’t Hof .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

van ’t Hof, R.J., Dall’Ara, E. (2019). Analysis of Bone Architecture in Rodents Using Micro-Computed Tomography. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_28

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_28

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation