Abstract
The 3T3-L1 murine preadipocyte cell line is a commonly used tool for analysis of the subcellular pathways involved in preadipocytic cell differentiation (a process also commonly known as adipogenesis). The major characteristic of adipogenesis is the intracellular accumulation of membrane-bound lipid droplets. Here, we describe methods used for the culture and transformation of these preadipocytes into mature adipocytes and quantification of intracellular lipid accumulation using the lipid specific dye, Oil Red O.
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Cave, E., Crowther, N.J. (2019). The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_25
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DOI: https://doi.org/10.1007/978-1-4939-8994-2_25
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8993-5
Online ISBN: 978-1-4939-8994-2
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