Abstract
The expression of Yamanaka factors (Oct3/4, Klf-4, Sox-2, c-Myc) can reprogram cancer cells to a pluripotent stage. This may cause the removal of their epigenetic memory and result in altered tumorigenicity. Various studies in the literature have shown that cancer cell reprogramming is a potential tool to study disease progression or discover novel therapeutic or diagnostic markers in cancer research. In this chapter, we aim to introduce the cancer cell reprogramming protocol in detail by using human melanocytes and melanoma cell lines, and Sendai viral vectors encoding Yamanaka factors have been used to reprogram cells. Representative results are discussed and important notes have been summarized in order to point out important steps during cancer cell reprogramming.
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References
Organization WH (2016) The top 10 causes of death. http://www.who.int/en/news-room/fact-sheets/detail/the-top-10-causes-of-death
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676
Siller R, Greenhough S, Park IH, Sullivan GJ (2013) Modelling human disease with pluripotent stem cells. Curr Gene Ther 13(2):99–110
Sng J, Lufkin T (2012) Emerging stem cell therapies: treatment, safety, and biology. Stem Cells Int 2012:521343. https://doi.org/10.1155/2012/521343
Dawson Mark A, Kouzarides T (2012) Cancer epigenetics: from mechanism to therapy. Cell 150(1):12–27
Koche RP, Smith ZD, Adli M, Gu H, Ku M, Gnirke A, Bernstein BE, Meissner A (2011) Reprogramming factor expression initiates widespread targeted chromatin remodeling. Cell Stem Cell 8(1):96–105
Jones PA, Baylin SB (2002) The fundamental role of epigenetic events in cancer. Nat Rev Genet 3:415. https://doi.org/10.1038/nrg816
Papp B, Plath K (2013) Epigenetics of reprogramming to induced pluripotency. Cell 152(6):1324–1343
Yilmazer A, de Lázaro I, Taheri H (2015) Reprogramming cancer cells: a novel approach for cancer therapy or a tool for disease-modeling? Cancer Lett 369(1):1–8
Yilmazer Aktuna A, Taheri H, Can A (2017) Efficient transduction of melanoma cells with Sendai viral vectors. Turk Hij Den Biyol Derg 74(2):113–120
Yilmazer A, Taheri H (2018) Reprogramming human melanocytes and melanoma cells with Yamanaka factors. Hacettepe J Biol Chem 46(1):35–41
Mochizuki H, Ohnuki Y, Kurosawa H (2011) Effect of glucose concentration during embryoid body (EB) formation from mouse embryonic stem cells on EB growth and cell differentiation. J Biosci Bioeng 111(1):92–97. https://doi.org/10.1016/j.jbiosc.2010.09.001
Okahara-Narita J, Umeda R, Nakamura S, Mori T, Noce T, Torii R (2012) Induction of pluripotent stem cells from fetal and adult cynomolgus monkey fibroblasts using four human transcription factors. Primates 53(2):205–213
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872. https://doi.org/10.1016/j.cell.2007.11.019
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Taheri, H., Cagin, U., Yilmazer, A. (2019). Reprogramming of Human Melanocytes and Melanoma Cells with Yamanaka Factors. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_24
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DOI: https://doi.org/10.1007/978-1-4939-8994-2_24
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