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Characterization of the db/db Mouse Model of Type 2 Diabetes

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Pre-Clinical Models

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1916))

Abstract

This chapter presents characterization of the db/db mouse model of type 2 diabetes. The protocol describes generation of the mice along with measurements of physical characteristics such as body weight and body composition, and behaviours including food and water consumption. Such a procedure can also be used for omics-based analyses of brain tissues to investigate the potential use of db/db mouse model in neuropsychiatric research. In addition, we present measurement of blood glucose and plasma insulin levels which tend to be elevated in this model and can modulated post treatment with anti-diabetic agents. All of these characteristics can be used for studying the pathophysiology and for monitoring response to potential therapeutics.

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Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil

    Paul C. Guest

  2. Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK

    Hassan Rahmoune

Authors
  1. Paul C. Guest
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  2. Hassan Rahmoune
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Corresponding author

Correspondence to Hassan Rahmoune .

Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Guest, P.C., Rahmoune, H. (2019). Characterization of the db/db Mouse Model of Type 2 Diabetes. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8994-2_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8993-5

  • Online ISBN: 978-1-4939-8994-2

  • eBook Packages: Springer Protocols

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