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Pre-Clinical Models pp 177–193Cite as

An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes Using Flow Cytometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1916))

Abstract

Various complications of diabetes are induced by the augmentation of chronic inflammation and attenuation of immunity. Leukocytes, which play major roles in inflammation and immune responses, are affected by the glycemic status and blood insulin level. In this chapter, we explain a method for analyzing the signal transduction pathway of leukocytes in peripheral blood. This method using flow cytometry can analyze a small amount of blood (50–100 μL/sample) without leukocyte purification. Thus, this procedure is useful for experiments using small-animal models of diabetes, such as mice and rats. We also introduce a new method for classifying intracellular signal transduction by combining the dispersibility level and the activation level of the signaling molecules.

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Acknowledgments

This work was supported by the Japan Society for the Promotion of Science Grant, No. 22590560, No. 80250744, No. 16K16294, and No. 17K11220001.

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Authors and Affiliations

  1. Department of Immunology, Faculty of Medicine, Yamagata University, Yamagata, Japan

    Yuji Takeda & Hironobu Asao

  2. Department of Enviromental and Preventive Medicine, Hyogo College of Medicine, Nishinomiya, Japan

    Ichiro Wakabayashi

Authors
  1. Yuji Takeda
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  2. Hironobu Asao
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  3. Ichiro Wakabayashi
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Corresponding author

Correspondence to Yuji Takeda .

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Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Takeda, Y., Asao, H., Wakabayashi, I. (2019). An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes Using Flow Cytometry. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8994-2_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8993-5

  • Online ISBN: 978-1-4939-8994-2

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