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Pre-Clinical Models pp 167–176Cite as

Experimental Model of HindLimb Suspension-Induced Skeletal Muscle Atrophy in Rodents

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1916))

Abstract

Due to the difficulty of performing research protocols that reproduce human skeletal muscle disuse conditions, an experimental animal model of “hindlimb suspension” (or hindlimb unloading) was developed. This method was created in the 1970s and utilizes rats and mice to mimic space flight and bed rest in humans. It provides an alternative to investigate mechanisms associated with skeletal muscle mass loss and interventions designed to attenuate atrophy induced by hindlimb unloading. The mentioned protocol also allows investigating quality of bones and changes in several physiological parameters such as blood pressure, heart rate, plasma or tissue lipid composition, and glycemia.

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Acknowledgments

The authors are grateful to José Roberto Mendonça for the excellent technical assistance. This research was supported by Becas Chile (CONICYT), Universidad de La Frontera, FAPESP, CNPq, CAPES, and Dean’s Office for Post-graduate Studies and Research of the Cruzeiro do Sul University.

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Authors and Affiliations

  1. Department of Internal Medicine, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile

    Gabriel Nasri Marzuca-Nassr

  2. School of Health Sciences, College of Health, Massey University, Auckland, New Zealand

    Kaio Fernando Vitzel

  3. Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil

    Gilson Masahiro Murata

  4. Faculty of Medical Sciences, School of Kinesiology, Universidad de Santiago de Chile, Santiago, Chile

    José Luis Márquez

  5. Interdisciplinary Post-Graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil

    Rui Curi

Authors
  1. Gabriel Nasri Marzuca-Nassr
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  2. Kaio Fernando Vitzel
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  3. Gilson Masahiro Murata
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  4. José Luis Márquez
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  5. Rui Curi
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Corresponding author

Correspondence to Gabriel Nasri Marzuca-Nassr .

Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Marzuca-Nassr, G.N., Vitzel, K.F., Murata, G.M., Márquez, J.L., Curi, R. (2019). Experimental Model of HindLimb Suspension-Induced Skeletal Muscle Atrophy in Rodents. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-8994-2_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8993-5

  • Online ISBN: 978-1-4939-8994-2

  • eBook Packages: Springer Protocols

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