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Maturation of a Human Oligodendrocyte Cell Line

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Pre-Clinical Models

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1916))

Abstract

Techniques such as the maturation and differentiation of cell lines and progenitor cells are important for the improvement and development of representative and relevant in vitro models. In this context, the following chapter proposes a maturation model of the MO3.13 cell line, aiming to contribute to a more robust and credible in vitro model of human oligodendrocytes. This may prove to be an important tool in the study of diseases related to dysfunctions in oligodendrocytes and demyelination, including schizophrenia and multiple sclerosis.

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References

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Acknowledgments

The authors thank FAPESP (Sao Paulo Research Foundation) for funding our research (grants 2013/08711-3, 2014/10068-4, and 2017/18242-1), the Serrapilheira Institute (grant number Serra-1709-16349), CNPq (The Brazilian National Council for Scientific and Technological Development, grant 302453/2017-2), and CAPES (Coordination for the Improvement of Higher Education Personnel, grant 1691474).

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Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Gabriela Seabra, Valéria de Almeida & Daniel Martins-de-Souza

  2. Center for Neurobiology, University of Campinas (UNICAMP), Campinas, Brazil

    Daniel Martins-de-Souza

  3. Instituto Nacional de Biomarcadores em Neuropsiquiatria (INBION), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Sao Paulo, Brazil

    Daniel Martins-de-Souza

Authors
  1. Gabriela Seabra
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  2. Valéria de Almeida
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  3. Daniel Martins-de-Souza
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Corresponding author

Correspondence to Daniel Martins-de-Souza .

Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Seabra, G., de Almeida, V., Martins-de-Souza, D. (2019). Maturation of a Human Oligodendrocyte Cell Line. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-8994-2_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8993-5

  • Online ISBN: 978-1-4939-8994-2

  • eBook Packages: Springer Protocols

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