Calpain pp 209-218 | Cite as

Experimental Manipulation of Calpain Activity In Vitro

  • Seiichi KitagawaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1915)


The calpain activity in cells can be experimentally manipulated in vitro by calpain inhibitors, and various types of calpain inhibitors such as peptide aldehydes and α-mercapto-acrylic acid derivatives are widely used as a valuable tool to elucidate the physiological and pathological roles of calpain. Here I describe the experimental procedures with calpain inhibitors, with human neutrophils being primarily used in this experiment. It should be noted that potent calpain inhibitors not only inhibit the calpain activity but also stimulate cell functions via direct activation of human formyl peptide receptors and/or other G protein-coupled receptors depending on the inhibitors used.

Key words

Calpain Calpain inhibitors Human formyl peptide receptors G protein-coupled receptors Migration Apoptosis 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Physiology, Graduate School of MedicineOsaka City UniversityOsakaJapan
  2. 2.Shitennoji Tamatsukurien ClinicOsakaJapan

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