Abstract
Protein complexes are responsible for major biological processes in cells. Identifying a protein’s interaction partners reveals many different aspects related to the target such as its function, regulation, and mechanisms of action. Co-immunoprecipitation (co-IP) followed by shotgun proteomics is a powerful technique for capturing and identifying endogenous protein complexes. Although it is a simple concept, performing the experiment can be challenging due to the number of steps and the diversity of available protocols. Here we present a certified and detailed protocol to perform co-IP experiments in cultured human oligodendrocytes cells.
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Acknowledgments
Research was funded by FAPESP (São Paulo Research Foundation, grants 2016/18715-4 and 2016/07948-8).
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Smith, B., Martins-de-Souza, D., Fioramonte, M. (2019). Using Co-immunoprecipitation and Shotgun Mass Spectrometry for Protein-Protein Interaction Identification in Cultured Human Oligodendrocytes. In: Odagaki, Y., Borroto-Escuela, D. (eds) Co-Immunoprecipitation Methods for Brain Tissue . Neuromethods, vol 144. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8985-0_4
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DOI: https://doi.org/10.1007/978-1-4939-8985-0_4
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