Abstract
RNA is mostly known for its role in protein synthesis, where it encodes information for protein sequence in its messenger RNA (mRNA) form (translation). Yet, RNA molecules regulate several cellular processes other than translation. Here, we present an overview of several mathematical models that help understanding and characterizing the role of noncoding RNA molecules (ncRNAs) in regulating gene expression and protein synthesis. First, we discuss relatively simple models where ncRNAs can modulate protein synthesis via targeting a mRNA. Then, we consider the case of feedback interactions between ncRNAs and their target proteins, and discuss several biological applications where these feedback architectures modulate a cellular phenotype and control the levels of intrinsic and extrinsic noise. Building from these simple circuit motifs, we examine feed-forward circuit motifs involving ncRNAs that generate precise spatial and temporal patterns of protein expression. Further, we investigate the competition between ncRNAs and other endogenous RNA molecules and show that the cross talk between coding and noncoding RNAs can form large genetic circuits that involve up to hundreds of chemical species. Finally, we discuss the role of ncRNAs in modulating cell-cell signaling pathways and therefore the dynamics of spatiotemporal pattern formation in a tissue.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Crick F (1970) Central dogma of molecular biology. Nature 227:561–563
Mattick JS, Makunin IV (2006) Non-coding RNA. Hum Mol Genet 15(Spec 1):17–29. https://doi.org/10.1093/hmg/ddl046
Eddy SR (2001) Non-coding RNA genes and the modern RNA world. Nat Rev Genet 2:919–929. https://doi.org/10.1038/35103511
Zhdanov VP (2011) Kinetic models of gene expression including non-coding RNAs. Phys Rep 500:1–42
Lai X, Wolkenhauer O, Vera J (2016) Understanding microRNA-mediated gene regulatory networks through mathematical modelling. Nucleic Acids Res 44:6019–6035
Ambros V (2004) The functions of animal microRNAs. Nature 431:350–355. https://doi.org/10.1038/nature02871
Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297. https://doi.org/10.1016/S0092-8674(04)00045-5
Neema A, Dasaradhi PVN, Asif Mohmmed PM, Bhatnagar RK, SKM (2004) RNA interference: biology, mechanism, and applications. Microbiol Mol Biol Rev 38:285–294. https://doi.org/10.1128/MMBR.67.4.657
Ma L, Bajic VB, Zhang Z (2013) On the classification of long non-coding RNAs. RNA Biol 10:924–933. https://doi.org/10.4161/rna.24604
Geisler S, Coller J (2013) RNA in unexpected places: long non-coding RNA functions in diverse cellular contexts. Nat Rev Mol Cell Biol 14:699–712. https://doi.org/10.1038/nrm3679
Gibb EA, Brown CJ, Lam WL (2011) The functional role of long non-coding RNA in human carcinomas. Mol Cancer 10:38. https://doi.org/10.1186/1476-4598-10-38
Gutschner T, Diederichs S (2012) The hallmarks of cancer: a long non-coding RNA point of view. RNA Biol 9:703–709. https://doi.org/10.4161/rna.20481
Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136:215–233. https://doi.org/10.1016/j.cell.2009.01.002
Ghildiyal M, Zamore PD (2009) Small silencing RNAs: an expanding universe. Nat Rev Genet 10:94–108. https://doi.org/10.1038/nrg2504
Yang E, van Nimwegen E, Zavolan M, Rajewsky N, Schroeder M, Magnasco M, Darnell JE (2003) Decay rates of human mRNAs: correlation with functional characteristics and sequence attributes. Genome Res 13:1863–1872. https://doi.org/10.1101/gr.1272403
Gottesman S (2005) Micros for microbes: non-coding regulatory RNAs in bacteria. Trends Genet 21:399–404. https://doi.org/10.1016/j.tig.2005.05.008
Storz G, Vogel J, Wassarman KM (2011) Regulation by small RNAs in bacteria: expanding frontiers. Mol Cell 43:880–891. https://doi.org/10.1016/j.molcel.2011.08.022
Mitarai N, Benjamin J-AM, Krishna S, Semsey S, Csiszovszki Z, Massé E, Sneppen K (2009) Dynamic features of gene expression control by small regulatory RNAs. Proc Natl Acad Sci U S A 106:10655–10659. https://doi.org/10.1073/pnas.0901466106
Massé E, Vanderpool CK, Gottesman S (2005) Effect of RyhB small RNA on global iron use in Escherichia coli. J Bacteriol 187:6962–6971. https://doi.org/10.1128/JB.187.20.6962-6971.2005
Veĉerek B, Moll I, Bläsi U (2007) Control of Fur synthesis by the non-coding RNA RyhB and iron-responsive decoding. EMBO J 26:965–975. https://doi.org/10.1038/sj.emboj.7601553
Hansen GT, Ahmad R, Hjerde E, Fenton CG, Willassen NP, Haugen P (2012) Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida. BMC Genomics 13:37. https://doi.org/10.1186/1471-2164-13-37
Semsey S, Andersson AMC, Krishna S, Jensen MH, Massé E, Sneppen K (2006) Genetic regulation of fluxes: iron homeostasis of Escherichia coli. Nucleic Acids Res 34:4960–4967. https://doi.org/10.1093/nar/gkl627
Mitarai N, Andersson AMC, Krishna S, Semsey S, Sneppen K (2007) Efficient degradation and expression prioritization with small RNAs. Phys Biol 4:164–171. https://doi.org/10.1088/1478-3975/4/3/003
Legewie S, Dienst D, Wilde A, Herzel H, Axmann IM (2008) Small RNAs establish delays and temporal thresholds in gene expression. Biophys J 95:3232–3238. https://doi.org/10.1529/biophysj.108.133819
Shimoni Y, Friedlander G, Hetzroni G, Niv G, Altuvia S, Biham O, Margalit H (2007) Regulation of gene expression by small non-coding RNAs: a quantitative view. Mol Syst Biol 3:1–9. https://doi.org/10.1038/msb4100181
Alon U (2007) Network motifs: theory and experimental approaches. Nat Rev Genet 8:450–461. https://doi.org/10.1038/nrg2102
Mangan S, Alon U (2003) Structure and function of the feed-forward loop network motif. Proc Natl Acad Sci U S A 100:11980–11985. https://doi.org/10.1073/pnas.2133841100
Levine E, Zhang Z, Kuhlman T, Hwa T (2007) Quantitative characteristics of gene regulation by small RNA. PLoS Biol 5:1998–2010. https://doi.org/10.1371/journal.pbio.0050229
Levine E, Hwa T (2008) Small RNAs establish gene expression thresholds. Curr Opin Microbiol 11:574–579. https://doi.org/10.1016/j.mib.2008.09.016
Levine E, Ben JE, Levine H (2007) Target-specific and global effectors in gene regulation by microRNA. Biophys J 93:L52–L54. https://doi.org/10.1529/biophysj.107.118448
Milo R, Jorgensen P, Moran U, Weber G, Springer M (2009) BioNumbers The database of key numbers in molecular and cell biology. Nucleic Acids Res 38:750–753. https://doi.org/10.1093/nar/gkp889
Swain PS, Elowitz MB, Siggia ED (2002) Intrinsic and extrinsic contributions to stochasticity in gene expression. Proc Natl Acad Sci 99:12795–12800. https://doi.org/10.1073/pnas.162041399
Elowitz MB, Levine AJ, Siggia ED, Swain PS (2002) Stochastic gene expression in a single cell. Science 297:1183–1186
Raser JM, O’Shea EK (2010) Noise in gene expression. Science 309:2010–2014. https://doi.org/10.1126/science.1105891
Schwanhüusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M (2011) Global quantification of mammalian gene expression control. Nature 473:337–342. https://doi.org/10.1038/nature10098
Jia Y, Liu W, Li A, Yang L, Zhan X (2009) Intrinsic noise in post-transcriptional gene regulation by small non-coding RNA. Biophys Chem 143:60–69. https://doi.org/10.1016/j.bpc.2009.04.001
Elgart V, Jia T, Kulkarni RV (2010) Applications of Little’s Law to stochastic models of gene expression. Phys Rev E Stat Nonlinear Soft Matter Phys 82:1–6. https://doi.org/10.1103/PhysRevE.82.021901
Elgart V, Jia T, Kulkarni R (2010) Quantifying mRNA synthesis and decay rates using small RNAs. Biophys J 98:2780–2784. https://doi.org/10.1016/j.bpj.2010.03.022
Zhdanov VP (2009) Conditions of appreciable influence of microRNA on a large number of target mRNAs. Mol BioSyst 5:638. https://doi.org/10.1039/b808095j
Jost D, Nowojewski A, Levine E (2013) Regulating the many to benefit the few: role of weak small RNA targets. Biophys J 104:1773–1782. https://doi.org/10.1016/j.bpj.2013.02.020
Shalgi R, Lieber D, Oren M, Pilpel Y (2007) Global and local architecture of the mammalian microRNA-transcription factor regulatory network. PLoS Comput Biol 3:1291–1304. https://doi.org/10.1371/journal.pcbi.0030131
Wu S, Huang S, Ding J, Zhao Y, Liang L, Liu T, Zhan R, He X (2010) Multiple microRNAs modulate p21Cip1/Waf1 expression by directly targeting its 3′ untranslated region. Oncogene 29:2302–2308. https://doi.org/10.1038/onc.2010.34
Lai X, Schmitz U, Gupta SK, Bhattacharya A, Kunz M, Wolkenhauer O, Vera J (2012) Computational analysis of target hub gene repression regulated by multiple and cooperative miRNAs. Nucleic Acids Res 40:8818–8834. https://doi.org/10.1093/nar/gks657
Lai X, Bhattacharya A, Schmitz U, Kunz M, Vera J, Wolkenhauer O (2013) A systems’ biology approach to study microrna-mediated gene regulatory networks. Biomed Res Int 2013:703849. https://doi.org/10.1155/2013/703849
Doench JG, Sharp PA (2004) Specificity of microRNA target selection in translational repression. Genes (Basel) 504:504–511. https://doi.org/10.1101/gad.1184404.species
Sætrom P, Heale BSE, Snøve O, Aagaard L, Alluin J, Rossi JJ (2007) Distance constraints between microRNA target sites dictate efficacy and cooperativity. Nucleic Acids Res 35:2333–2342. https://doi.org/10.1093/nar/gkm133
Schmitz U, Lai X, Winter F, Wolkenhauer O, Vera J, Gupta SK (2014) Cooperative gene regulation by microRNA pairs and their identification using a computational workflow. Nucleic Acids Res 42:7539–7552. https://doi.org/10.1093/nar/gku465
Lu M, Jolly MK, Levine H, Onuchic JN, Ben-Jacob E (2013) MicroRNA-based regulation of epithelial-hybrid-mesenchymal fate determination. Proc Natl Acad Sci U S A 110:18174–18179. https://doi.org/10.1073/pnas.1318192110
Vasudevan S, Tong Y, Steitz JA (2007) Switching from repression to activation: MicroRNAs can up-regulate translation. Science 318:1931–1934
Ghosh T, Soni K, Scaria V, Halimani M, Bhattacharjee C, Pillai B (2008) MicroRNA-mediated up-regulation of an alternatively polyadenylated variant of the mouse cytoplasmic β-actin gene. Nucleic Acids Res 36:6318–6332. https://doi.org/10.1093/nar/gkn624
Ma F, Liu X, Li D, Wang P, Li N, Lu L, Cao X (2010) MicroRNA-466l upregulates IL-10 expression in TLR-triggered macrophages by antagonizing RNA-binding protein tristetraprolin-mediated IL-10 mRNA degradation. J Immunol 184:6053–6059. https://doi.org/10.4049/jimmunol.0902308
Gokhale SA, Gadgil CJ (2012) Analysis of miRNA regulation suggests an explanation for ‘unexpected’ increase in target protein levels. Mol BioSyst 8:760–765. https://doi.org/10.1039/C1MB05368J
Zhdanov VP (2010) ncRNA-mediated bistability in the synthesis of hundreds of distinct mRNAs and proteins. Phys A Stat Mech Appl 389:887–890. https://doi.org/10.1016/j.physa.2009.11.028
Zhdanov VP (2010) Effect of non-coding RNA on bistability and oscillations in the mRNA-protein interplay. Biophys Rev Lett 05:89–107
Zhdanov VP (2006) Transient stochastic bistable kinetics of gene transcription during the cellular growth. Chem Phys Lett 424:394–398. https://doi.org/10.1016/j.cplett.2006.05.024
Tian XJ, Zhang H, Xing J (2013) Coupled reversible and irreversible bistable switches underlying TGFβ-induced epithelial to mesenchymal transition. Biophys J 105:1079–1089. https://doi.org/10.1016/j.bpj.2013.07.011
Nieto MA, Huang RY, Jackson RA, Thiery JP (2016) EMT: 2016. Cell 166:21–45
Jolly MK, Boareto M, Huang B, Jia D, Lu M, Ben-Jacob E, Onuchic JN, Levine H (2015) Implications of the hybrid epithelial/mesenchymal phenotype in metastasis. Front Oncol 5:155. https://doi.org/10.3389/fonc.2015.00155
Yang X, Lin X, Zhong X, Kaur S, Li N, Liang S, Lassus H, Wang L, Katsaros D, Montone K, Zhao X, Zhang Y, Bützow R, Coukos G, Zhang L (2010) Double-negative feedback loop between reprogramming factor LIN28 and microRNA let-7 regulates aldehyde dehydrogenase 1-positive cancer stem cells. Cancer Res 70:9463–9472. https://doi.org/10.1158/0008-5472.CAN-10-2388
Jolly MK, Huang B, Lu M, Mani SA, Levine H, Ben-Jacob E (2014) Towards elucidating the connection between epithelial--mesenchymal transitions and stemness. J R Soc Interface 11:20140962
Jolly MK, Jia D, Boareto M, Mani SA, Pienta KJ, Ben-Jacob E, Levine H (2015) Coupling the modules of EMT and stemness: a tunable ‘stemness window’model. Oncotarget 6:25161–25174
Hafner M, Max KEA, Bandaru P, Morozov P, Gerstberger S, Brown M, Molina H, Tuschl T (2013) Identification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition. RNA 19:613–626. https://doi.org/10.1261/rna.036491.112
Wilbert ML, Huelga SC, Kapeli K, Stark TJ, Liang TY, Chen SX, Yan BY, Nathanson JL, Hutt KR, Lovci MT, Kazan H, Vu AQ, Massirer KB, Morris Q, Hoon S, Yeo GW (2012) LIN28 binds messenger RNAs at GGAGA motifs and regulates splicing factor abundance. Mol Cell 48:195–206. https://doi.org/10.1016/j.molcel.2012.08.004
Zisoulis DG, Kai ZS, Chang RK, Pasquinelli AE (2012) Autoregulation of microRNA biogenesis by let-7 and Argonaute. Nature 486:541–544. https://doi.org/10.1038/nature11134
Emmrich S, Pützer BM (2010) Checks and balances: E2F – MicroRNA crosstalk in cancer control. Cell Cycle 9:2555–2567. https://doi.org/10.4161/cc.9.13.12061
Concepcion CP, Bonetti C, Ventura A (2012) The MicroRNA-17-92 family of MicroRNA clusters in development and disease. Cancer J (United States) 18:262–267. https://doi.org/10.1097/PPO.0b013e318258b60a
Aguda BD, Kim Y, Piper-Hunter MG, Friedman A, Marsh CB (2008) MicroRNA regulation of a cancer network: consequences of the feedback loops involving miR-17-92, E2F, and Myc. Proc Natl Acad Sci 105:19678–19683. https://doi.org/10.1073/pnas.0811166106
Zhang H, Chen Y, Chen Y (2012) Noise propagation in gene regulation networks involving interlinked positive and negative feedback loops. PLoS One 7:1–8. https://doi.org/10.1371/journal.pone.0051840
Li Y, Li Y, Zhang H, Chen Y (2011) Microrna-mediated positive feedback loop and optimized bistable switch in a cancer network involving miR-17-92. PLoS One 6:2–10. https://doi.org/10.1371/journal.pone.0026302
Giampieri E, Remondini D, de Oliveira L, Castellani G, Lió P (2011) Stochastic analysis of a miRNA-protein toggle switch. Mol BioSyst 7:2796–2803. https://doi.org/10.1039/c1mb05086a
Tsang J, Zhu J, van Oudenaarden A (2007) MicroRNA-mediated feedback and feedforward loops are recurrent network motifs in mammals. Mol Cell 26:753–767. https://doi.org/10.1016/j.molcel.2007.05.018
Re A, Cora D, Taverna D, Caselle M (2009) Genome-wide survey of MicroRNA – transcription factor feed-forward regulatory circuits in human. BMC Bioinformatics 5:51. https://doi.org/10.1039/b900177h
Wall ME, Dunlop MJ, Hlavacek WS (2005) Multiple functions of a feed-forward-loop gene circuit. J Mol Biol 349:501–514. https://doi.org/10.1016/j.jmb.2005.04.022
Herranz H, Cohen SM (2010) MicroRNAs and gene regulatory networks: managing the impact of noise in biological systems. Genes Dev 24:1339–1344. https://doi.org/10.1101/gad.1937010
Shalgi R, Brosh R, Oren M, Pilpel Y, Rotter V (2009) Coupling transcriptional and post-transcriptional miRNA regulation in the control of cell fate. Aging (Albany NY) 1:762–770
Kobayashi K, Sakurai K, Hiramatsu H, Inada KI, Shiogama K, Nakamura S, Suemasa F, Kobayashi K, Imoto S, Haraguchi T, Ito H, Ishizaka A, Tsutsumi Y, Iba H (2015) The miR-199a/Brm/EGR1 axis is a determinant of anchorage-independent growth in epithelial tumor cell lines. Sci Rep 5:8428. https://doi.org/10.1038/srep08428
Taulli R, Loretelli C, Pandolfi PP (2013) From pseudo-ceRNAs to circ-ceRNAs: a tale of cross-talk and competition. Nat Struct Mol Biol 20:541–543. https://doi.org/10.1038/nsmb2580
Tay Y, Rinn J, Pandolfi PP (2014) The multilayered complexity of ceRNA crosstalk and competition. Nature 505:344–352. https://doi.org/10.1038/nature12986
Kartha RV, Subramanian S (2014) Competing endogenous RNAs (ceRNAs): new entrants to the intricacies of gene regulation. Front Genet 5:1–9. https://doi.org/10.3389/fgene.2014.00008
Ebert MS, Sharp PA (2012) Roles for MicroRNAs in conferring robustness to biological processes. Cell 149:505–524. https://doi.org/10.1016/j.cell.2012.04.005
Salmena L, Poliseno L, Tay Y, Kats L, Pandolfi PP (2011) A ceRNA hypothesis: the rosetta stone of a hidden RNA language? Cell 146:353–358. https://doi.org/10.1016/j.cell.2011.07.014
Ala U, Karreth FA, Bosia C, Pagnani A, Taulli R, Leopold V, Tay Y, Provero P, Zecchina R, Pandolfi PP (2013) Integrated transcriptional and competitive endogenous RNA networks are cross-regulated in permissive molecular environments. Proc Natl Acad Sci U S A 110:7154–7159. https://doi.org/10.1073/pnas.1222509110
Bosia C, Pagnani A, Zecchina R (2013) Modelling competing endogenous RNA networks. PLoS One 8:e66609. https://doi.org/10.1371/journal.pone.0066609
Martirosyan A, Figliuzzi M, Marinari E, De Martino A (2016) Probing the limits to MicroRNA-mediated control of gene expression. PLoS Comput Biol 12:1–23. https://doi.org/10.1371/journal.pcbi.1004715
Gérard C, Novák B (2013) microRNA as a potential vector for the propagation of robustness in protein expression and oscillatory dynamics within a ceRNA network. PLoS One 8:e83372. https://doi.org/10.1371/journal.pone.0083372
Pedraza JM, van Oudenaarden A (2005) Noise propagation in gene networks. Science 307:1965–1970
Skalsky RL, Cullen BR (2010) Viruses, microRNAs, and host interactions. Annu Rev Microbiol 64:123–141. https://doi.org/10.1146/annurev.micro.112408.134243
Grundhoff A, Sullivan CS (2011) Virus-encoded microRNAs. Virology 411:325–343. https://doi.org/10.1016/j.virol.2011.01.002
Cullen BR (2009) Viral and cellular messenger RNA targets of viral microRNAs. Nature 457:421–425. https://doi.org/10.1038/nature07757
McCaskill J, Praihirunkit P, Sharp PM, Buck AH (2015) RNA-mediated degradation of microRNAs: a widespread viral strategy? RNA Biol 12:579–585. https://doi.org/10.1080/15476286.2015.1034912
Luna JM, Scheel TKH, Danino T, Shaw KS, Mele A, Fak JJ, Nishiuchi E, Takacs CN, Catanese MT, De Jong YP, Jacobson IM, Rice CM, Darnell RB (2015) Hepatitis C virus RNA functionally sequesters miR-122. Cell 160:1099–1110. https://doi.org/10.1016/j.cell.2015.02.025
Bray SJ (2006) Notch signalling: a simple pathway becomes complex. Nat Rev Mol Cell Biol 7:678–689. https://doi.org/10.1038/nrm2009
Andersson ER, Sandberg R, Lendahl U (2011) Notch signaling: simplicity in design, versatility in function. Development 138:3593–3612
Bolos V, Grego-Bessa J, De La Pompa JL (2007) Notch signaling in development and cancer. Endocr Rev 28:339–363. https://doi.org/10.1210/er.2006-0046
Gerdes HH, Rustom A, Wang X (2013) Tunneling nanotubes, an emerging intercellular communication route in development. Mech Dev 130:381–387. https://doi.org/10.1016/j.mod.2012.11.006
Gerdes HH, Bukoreshtliev NV, Barroso JFV (2007) Tunneling nanotubes: a new route for the exchange of components between animal cells. FEBS Lett 581:2194–2201. https://doi.org/10.1016/j.febslet.2007.03.071
Théry C, Zitvogel L, Amigorena S (2002) Exosomes: composition, biogenesis and function. Nat Rev Immunol 2:569–579. https://doi.org/10.1038/nri855
Raposo G, Stoorvogel W (2013) Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 200:373–383. https://doi.org/10.1083/jcb.201211138
Shaya O, Sprinzak D (2011) From Notch signaling to fine-grained patterning: modeling meets experiments. Curr Opin Genet Dev 21:732–739
Beatus P, Lendahl U (1998) Notch and neurogenesis. J Neurosci Res 54:125–136. https://doi.org/10.1002/(SICI)1097-4547(19981015)54:2<125::AID-JNR1>3.0.CO;2-G
Boareto M, Jolly MK, Lu M, Onuchic JN, Clementi C, Ben-Jacob E (2015) Jagged--Delta asymmetry in Notch signaling can give rise to a Sender/Receiver hybrid phenotype. Proc Natl Acad Sci U S A 112:402–409
Jolly MK, Boareto M, Lu M, Onuchic JN, Clementi C, Ben-Jacob E, Jose’N O, Clementi C, Ben-Jacob E (2015) Operating principles of Notch--Delta--Jagged module of cell--cell communication. New J Phys 17:55021
Hartman BH, Reh TA, Bermingham-McDonogh O (2010) Notch signaling specifies prosensory domains via lateral induction in the developing mammalian inner ear. Proc Natl Acad Sci U S A 107:15792–15797
Chen JS, Gumbayan AM, Zeller RW, Mahaffy JM (2014) An expanded Notch-Delta model exhibiting long-range patterning and incorporating MicroRNA regulation. PLoS Comput Biol 10:e1003655. https://doi.org/10.1371/journal.pcbi.1003655
Goodfellow M, Phillips NE, Manning C, Galla T, Papalopulu N (2014) MicroRNA input into a neural ultradian oscillator controls emergence and timing of alternative cell states. Nat Commun 5:1–10. https://doi.org/10.1038/ncomms4399
Roese-Koerner B, Stappert L, Brüstle O (2017) Notch/Hes signaling and miR-9 engage in complex feedback interactions controlling neural progenitor cell proliferation and differentiation. Neurogenesis 4:e1313647. https://doi.org/10.1080/23262133.2017.1313647
Boareto M, Jolly MK, Goldman A, Pietilä M, Mani SA, Sengupta S, Ben-Jacob E, Levine H, Jose’N O (2016) Notch-Jagged signalling can give rise to clusters of cells exhibiting a hybrid epithelial/mesenchymal phenotype. J R Soc Interface 13:20151106
Fabisiewicz A, Grzybowska E, Grybowska E (2017) CTC clusters in cancer progression and metastasis. Med Oncol 34:12
Bocci F, Jolly MK, Tripathi SC, Aguilar M, Onuchic N, Hanash SM, Levine H, Levine H (2017) Numb prevents a complete epithelial – mesenchymal transition by modulating Notch signalling. J R Soc Interface 14:20170512
Jolly MK, Tripathi SC, Jia D, Mooney SM, Celiktas M, Hanash SM, Mani SA, Pienta KJ, Ben-Jacob E, Levine H (2016) Stability of the hybrid epithelial/mesenchymal phenotype. Oncotarget 7:27067–27084. https://doi.org/10.18632/oncotarget.8166
Bocci F, Jolly MK, George J, Levine H, Onuchic JN (2018) A mechanism-based computational model to capture the interconnections among epithelial-mesenchymal transition, cancer stem cells and Notch-Jagged signaling. Oncotarget 9:29906–29920. https://doi.org/10.1101/314187
Bocci F, Gearhart-Serna L, Boareto M, Ribeiro M, Ben-Jacob E, Devi GR, Levine H, Onuchic JN, Jolly MK (2018) Towards understanding cancer stem cell heterogeneity in the tumor microenvironment. BiorXiv https://doi.org/10.1101/408823
Bocci F, Levine H, Onuchic JN, Jolly MK (2018) Deciphering the dynamics of Epithelial-Mesenchymal Transition and Cancer Stem Cells in tumor progression. Arxiv https://arxiv.org/pdf/1808.09113
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Bocci, F., Jolly, M.K., Levine, H., Onuchic, J.N. (2019). Quantitative Characteristic of ncRNA Regulation in Gene Regulatory Networks. In: Lai, X., Gupta, S., Vera, J. (eds) Computational Biology of Non-Coding RNA. Methods in Molecular Biology, vol 1912. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8982-9_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8982-9_14
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8981-2
Online ISBN: 978-1-4939-8982-9
eBook Packages: Springer Protocols