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Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy

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Hepatitis C Virus Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1911))

Abstract

Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.

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Acknowledgments

We would like to thank Ricardo Henriques and Lucas Walker for advice and technical support. JG is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (107653/Z/15/Z). PMP is supported by a Biotechnology and Biological Sciences Research Council grant (BB/M022374/1). CJ is a Commonwealth Scholar, funded by the UK government. The imaging systems used in this work were funded by the Medical Research Council (MR/K015826/1).

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Authors and Affiliations

  1. Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, London, UK

    Pedro Matos Pereira & Caron Jacobs

  2. Division of Infection and Immunity, Institute of Immunity and Transplantation, University College London, London, UK

    Joe Grove

Authors
  1. Pedro Matos Pereira
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  2. Caron Jacobs
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  3. Joe Grove
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Corresponding author

Correspondence to Joe Grove .

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Editors and Affiliations

  1. Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA

    Mansun Law

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Pereira, P.M., Jacobs, C., Grove, J. (2019). Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy. In: Law, M. (eds) Hepatitis C Virus Protocols . Methods in Molecular Biology, vol 1911. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8976-8_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8976-8_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8975-1

  • Online ISBN: 978-1-4939-8976-8

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