Microchip Isotachophoresis: Analysis of Pharmaceuticals

  • Marián MasárEmail author
  • Jasna Hradski
Part of the Methods in Molecular Biology book series (MIMB, volume 1906)


Microchip isotachophoresis (μITP) is a miniaturized version of conventional isotachophoresis (ITP) characterized by low sample and buffer consumption and reduced waste production. μITP with universal conductivity detection is suitable for quantitative analysis of relatively simplified samples that contain analyte(s) at relatively high concentration, e.g., pharmaceutical preparations. Here we describe in detail a principle of μITP in terms of reaching highly precise results. A practical use of μITP is shown on the analyses of various pharmaceutical preparations for content of major constituents including active pharmaceutical ingredients as well as pharmaceutical counterions. The pharmaceuticals are treated only minimally prior to the ITP run on a microchip with coupled channels and sample injection channel with 0.9 μL volume. Developed method is suitable for rapid (analysis time up to 10 min), precise (less than 1% RSD of analyte zone length), and accurate (recovery of 98–101%) determination of major pharmaceutical ingredients using a method of internal standard for data evaluation.

Key words

Microchip isotachophoresis Microchip with coupled channels Contact conductivity detection Pharmaceuticals N-acetylcysteine Buserelin acetate 



This work was supported by the Slovak Research and Development Agency (APVV-17-0318) and the Slovak Grant Agency for Science (VEGA 1/0340/15). The financial support of Merck (Darmstadt, Germany) is also acknowledged.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Analytical Chemistry, Faculty of Natural SciencesComenius University in BratislavaBratislavaSlovakia

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