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Micellar Electrokinetic Chromatography

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Microfluidic Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1906))

Abstract

Micellar electrokinetic chromatography (MEKC) is a mode of capillary electrophoresis that allows for the separation of neutral molecules in an electric field. Typically, neutral molecules move with electroosmotic flow (EOF) or bulk flow during electrophoretic separations resulting in no temporal resolution between mixtures of neutral analytes. Inclusion of surfactant micelles in the separation buffer allows for the separation of neutral analytes from one another through association with the micelle. Here we outline the implementation of MEKC for the separation of neutral molecules using a mixture of nitroaromatic explosives and their degradation products serving as a test analyte mixture.

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Author information

Authors and Affiliations

  1. Chemistry Division, U.S. Naval Research Laboratory, Washington, DC, USA

    Braden C. Giordano & Greg E. Collins

  2. Chemistry Department , United States Naval Academy, Annapolis, MD, USA

    Ronald Siefert

Authors
  1. Braden C. Giordano
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  2. Ronald Siefert
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  3. Greg E. Collins
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Corresponding author

Correspondence to Braden C. Giordano .

Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Wyoming, Laramie, WY, USA

    Debashis Dutta

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Giordano, B.C., Siefert, R., Collins, G.E. (2019). Micellar Electrokinetic Chromatography. In: Dutta, D. (eds) Microfluidic Electrophoresis. Methods in Molecular Biology, vol 1906. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8964-5_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8964-5_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8963-8

  • Online ISBN: 978-1-4939-8964-5

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