Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection

  • Lucas Paines Bressan
  • Dosil Pereira de Jesus
  • Dulan Bandara Gunasekara
  • Susan Marie Lunte
  • José Alberto Fracassi da SilvaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1906)


Microchip electrophoresis is a versatile separation technique. Electrochemical detection is suitable to apply to microdevices due to its easy integration to the fabrication process and good sensitivity and selectivity. Here we describe the procedures to prepare Pt band electrodes deposited on glass to couple to polydimethylsiloxane (PDMS) microchips aiming the separation and detection of nitrite using an isolated potentiostat.

Key words

Amperometric detection Polydimethylsiloxane Isolated potentiostat Gated injection Reactive nitrogen species 



This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grants 444514/2014-7 and 465389/2014-7, Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) grants 2014/50867-3 and 2013/22127-2, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) grant CAPES-COFECUB 802-14, and National Institutes of Health (NIH). Authors thank Instituto Nacional de Ciência e Tecnologia em Bioanalítica (INCTBio).


  1. 1.
    Patabadige DEW, Jia S, Sibbitts J, Sadeghi J, Sellens K, Culbertson CT (2016) Micro total analysis systems: fundamental advances and applications. Anal Chem 88:320–338CrossRefGoogle Scholar
  2. 2.
    Reyes DR, Iossifidis D, Auroux P-A, Manz A (2002) Micro total analysis systems. 1. Introduction, theory, and technology. Anal Chem 74:2623–2636CrossRefGoogle Scholar
  3. 3.
    Culbertson CT, Mickleburgh T, Stewart-James SA, Sellens KA, Pressnall M (2014) Micro total analysis systems: fundamental advances and biological applications. Anal Chem 86:95–118CrossRefGoogle Scholar
  4. 4.
    Kovarik ML, Ornoff DM, Melvin AT, Dobes NC, Wang Y, Dickinson AJ, Gach PC, Shah PK, Allbritton NL (2013) Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field. Anal Chem 85:451–472CrossRefGoogle Scholar
  5. 5.
    Kovarik ML, Gach PC, Ornoff DM, Wang YL, Balowski J, Farrag L, Allbritton NL (2012) Micro total analysis systems for cell biology and biochemical assays. Anal Chem 84:516–540CrossRefGoogle Scholar
  6. 6.
    Gunasekara DB, Hulvey MK, Lunte SM, da Silva JAF (2012) Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts. Anal Bioanal Chem 403:2377–2384CrossRefGoogle Scholar
  7. 7.
    Gunasekara DB, Hulvey MK, Lunte SM (2011) In-channel amperometric detection for microchip electrophoresis using a wireless isolated potentiostat. Electrophoresis 32:832–837CrossRefGoogle Scholar
  8. 8.
    Gunasekara DB, Siegel JM, Caruso G, Hulvey MK, Lunte SM (2014) Microchip electrophoresis with amperometric detection method for profiling cellular nitrosative stress markers. Analyst 139:3265–3273CrossRefGoogle Scholar
  9. 9.
    Hulvey MK, Frankenfeld CN, Lunte SM (2010) Separation and detection of peroxynitrite using microchip electrophoresis with Amperometric detection. Anal Chem 82:1608–1611CrossRefGoogle Scholar
  10. 10.
    Moreira NH, Almeida ALJ, Piazzeta MHO, de Jesus DP, Deblire A, Gobbi AL, da Silva JAF (2009) Fabrication of a multichannel PDMS/glass analytical microsystem with integrated electrodes for amperometric detection. Lab Chip 9:115–121CrossRefGoogle Scholar
  11. 11.
    Zhang G, Du W, Liu B-F, Hisamoto H, Terabe S (2007) Characterization of electrokinetic gating valve in microfluidic channels. Anal Chim Acta 584:129–135CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Lucas Paines Bressan
    • 1
  • Dosil Pereira de Jesus
    • 1
  • Dulan Bandara Gunasekara
    • 2
  • Susan Marie Lunte
    • 3
  • José Alberto Fracassi da Silva
    • 1
    Email author
  1. 1.Chemistry InstituteState University of CampinasCampinasBrazil
  2. 2.Department of ChemistryUniversity of North Carolina at Chapel HillChapel HillUSA
  3. 3.Ralph N. Adams Institute for Bioanalytical ChemistryUniversity of KansasLawrenceUSA

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