Sample Preconcentration Protocols in Microfluidic Electrophoresis

  • Fumihiko KitagawaEmail author
  • Koji Otsuka
Part of the Methods in Molecular Biology book series (MIMB, volume 1906)


Electrophoretic on-line sample preconcentration techniques in microfluidic channels improve the sensitivity prior to the separation. Among various techniques, the most important field-amplified sample stacking and sweeping on cross-channel microchips are demonstrated. As a novel microfluidic preconcentration approach, a large-volume sample stacking with electroosmotic flow pump (LVSEP) on straight-channel chips is also presented, which can omit a complicated voltage program for sample injection processes. In this chapter, we describe how to prepare and how to run these on-line sample preconcentration methods in microchip electrophoresis.

Key words

Microchip electrophoresis On-line sample preconcentration Field-amplified sample stacking Sweeping LVSEP 



We would like to thank Dr. Kenji Sueyoshi (Osaka Prefecture University, Japan) and Dr. Takayuki Kawai (RIKEN, Japan) for collaborating the sweeping and LVSEP experiments, respectively. This work was supported in part by the Grant-in-Aid for Scientific Research (C) (No. 24550090 and 15K05527) from the Japan Society for the Promotion of Science (JSPS). This research was also supported by SENTAN, JST.


  1. 1.
    Jacobson SC, Koutny LB, Hergenroder R, Moore AW, Ramsey JM (1994) Microchip capillary electrophoresis with an integrated postcolumn reactor. Anal Chem 66:3472–3476CrossRefGoogle Scholar
  2. 2.
    Burgi DS, Chien RL (1991) Optimization in sample stacking for high-performance capillary electrophoresis. Anal Chem 63:2042–2047CrossRefGoogle Scholar
  3. 3.
    He Y, Lee HK (1999) Large-volume sample stacking in acidic buffer for analysis of small organic and inorganic anions by capillary electrophoresis. Anal Chem 71:995–1001CrossRefGoogle Scholar
  4. 4.
    Kawai T, Sueyoshi K, Kitagawa F, Otsuka K (2010) Microchip electrophoresis of oligosaccharides using large-volume sample stacking with electroosmotic flow pump in single channel. Anal Chem 82:6504–6511CrossRefGoogle Scholar
  5. 5.
    Kitagawa F, Kawai T, Otsuka K (2013) On-line sample preconcentration by large volume sample stacking with an electroosmotic flow pump (LVSEP) in microscale electrophoresis. Anal Sci 29:1129–1139CrossRefGoogle Scholar
  6. 6.
    Kitagawa F, Kinami S, Takegawa Y, Nukatsuka I, Sueyoshi K, Kawai T, Otsuka K (2017) On-line coupling of sample preconcentration by LVSEP with gel electrophoretic separation on T-channel chips. Electrophoresis 38:380–386CrossRefGoogle Scholar
  7. 7.
    Quirino JP, Terabe S (1998) Exceeding 5000-fold concentration of dilute analytes in micellar electrokinetic chromatography. Science 282:465–468CrossRefGoogle Scholar
  8. 8.
    Duffy DC, McDonald JC, Schueller OJA, Whitesides GM (1998) Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Anal Chem 70:4974–4984CrossRefGoogle Scholar
  9. 9.
    Wu D, Luo Y, Zhou X, Dai Z, Lin B (2005) Multilayer poly(vinyl alcohol)-adsorbed coating on poly(dimethylsiloxane) microfluidic chips for biopolymer separation. Electrophoresis 26:211–218CrossRefGoogle Scholar
  10. 10.
    Kitagawa F, Nakagawara S, Nukatsuka I, Hori Y, Sueyoshi K, Otsuka K (2015) Simple and rapid immobilization of coating polymers on poly(dimethyl siloxane)-glass hybrid microchips by a vacuum-drying method. Anal Sci 31:1171–1175CrossRefGoogle Scholar
  11. 11.
    Jacobson SC, Hergenroder R, Moore AW, Ramsey JM (1994) Precolumn reactions with electrophoretic analysis integrated on a microchip. Anal Chem 66:4127–4132CrossRefGoogle Scholar
  12. 12.
    Quirino JP, Terabe S (1999) Sweeping of analyte zones in electrokinetic chromatography. Anal Chem 71:1638–1644CrossRefGoogle Scholar
  13. 13.
    Kawai T, Ito J, Sueyoshi K, Kitagawa F, Otsuka K (2012) Electrophoretic analysis of cations using large-volume sample stacking with an electroosmotic flow pump using capillaries coated with neutral and cationic polymers. J Chromatogr A 1267:65–73CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Frontier Materials Chemistry, Graduate School of Science and TechnologyHirosaki UniversityHirosakiJapan
  2. 2.Department of Material Chemistry, Graduate School of EngineeringKyoto UniversityKyotoJapan

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