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Tobacco Rattle Virus-Induced Gene Silencing in Cotton

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Transgenic Cotton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1902))

Abstract

Virus-induced gene silencing (VIGS), as a tool for plant reverse genetics, has been widely used in cotton for target gene function analysis. Compared with genetically transformed plants, the target gene expression level is reduced in the newly emerged leaves and can carry out phenotype identification after a few weeks of Agrobacterium infiltration. In this chapter, we describe a detailed protocol for Agrobacterium-mediated TRV-VIGS system for cotton gene function studies, with focus on designing primers, constructing TRV-target gene vectors via homologous recombination method, preparing and infiltrating Agrobacterium with TRV-VIGS, and identifying target gene silencing.

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Acknowledgment

This work was financially supported by the “Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (TAPP) (PPZY2015A059)” (to CC) and the Cotton Incorporated (to BZ). The binary vectors of pTRV1 and pTRV2 were generously provided by Dr. Libo Shan of Texas A&M University (College Station, TX, USA).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics & Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu, China

    Caiping Cai & Wangzhen Guo

  2. Department of Biology, East Carolina University, Greenville, NC, USA

    Caiping Cai & Baohong Zhang

  3. College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, China

    Xinyu Wang

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  1. Caiping Cai
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  2. Xinyu Wang
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  3. Baohong Zhang
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  4. Wangzhen Guo
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Correspondence to Wangzhen Guo .

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Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

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Cai, C., Wang, X., Zhang, B., Guo, W. (2019). Tobacco Rattle Virus-Induced Gene Silencing in Cotton. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 1902. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8952-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8952-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8951-5

  • Online ISBN: 978-1-4939-8952-2

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