Skip to main content
SpringerLink
Log in
Book cover

Transgenic Cotton pp 293–305Cite as

Development of Virus Resistance Transgenic Cotton Using Cotton Leaf Curl Virus Antisense ßC1 Gene

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1902))

Abstract

Cotton (Gossypium hirsutum L.) is the most economically important crop in the world and produced 90% of the total natural cellulose fiber which is utilized to make cotton fabrics. The production of cotton is affected by many several diseases, and among them, viral disease, especially leaf curl, is the most destructive disease caused by a begomovirus transmitted by whiteflies vector. Plant biotechnology has provided an opportunity to develop transgenic plant with variable traits against biotic and abiotic stress such as resistance against pathogens, yield, quality, and salinity. Transgenic cotton (Gossypium hirsutum L., cv. Coker 312) plants were raised against leaf curl disease using bC1 gene in antisense orientation through Agrobacterium-mediated transformation somatic embryogenesis system. In this chapter, a standardized protocol will be given to raise virus resistance transgenic cotton.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Wendel JF, Cronn RC (2003) Polyploidy and the evolutionary history of cotton. Adv Agron 78:139–186

    Article  Google Scholar 

  2. Duncan DR, Zehr UB (2010) (ed.), Cotton, Biotechnology in Agriculture and Forestry 65, Springer-Verlag, Berlin Heidelberg

    Google Scholar 

  3. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204

    Article  CAS  Google Scholar 

  4. Davidonis GH, Hamilton RH (1983) Plant regeneration from callus tissue of Gossypium hirsutum L. Plant Sci Lett 32:89–93

    Article  CAS  Google Scholar 

  5. Umbeck P, Johnson G, Barton K, Swain W (1987) Genetically transformed cotton (Gossypium hirsutum L.) plants. Biotechnology 5:263–266

    CAS  Google Scholar 

  6. Firoozabady E, DeBoer DL, Merlo DJ, Halk EL, Amerson LN, Rashka KE, Murray EE (1987) Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants. Plant Mol Biol 10:105–116

    Article  CAS  Google Scholar 

  7. Wu SJ, Wang HH, Li FF, Chen TZ, Zhang J, Jiang JJ, Ding Y, Guo WZ, Zhang TZ (2008) Enhanced Agrobacterium-mediated transformation of embryogenic calli of upland cotton via efficient selection and timely subculture of somatic embryos. Plant Mol Biol Rep 26:174–185

    Article  CAS  Google Scholar 

  8. Jin S, Zhang X, Liang S, Nie Y, Guo X, Huang C (2005) Factors affecting transformation efficiency of embryogenic callus of upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens. Plant Cell Tissue Organ Cult 81:229–237

    Article  CAS  Google Scholar 

  9. Noman A, Rohina B, Muhammad A, Sumera A, Wasif I, Madiha Z, Sara Z, Shahbaz K, Waqar I, Muhammad A (2016) Success of transgenic cotton (Gossypium hirsutum L.): Fiction or reality? Cog Food Agric 2:1207844

    Google Scholar 

  10. Rathore KS, Sunilkumar G, Cantrell RG, Reding HK, Hague S (2009) Compendium of transgenic crop plants. Transgenic sugar, tuber and fiber crops. Wiley Online Library, Hoboken, NJ

    Google Scholar 

  11. Rahman M, Rao AQ, Batool F, Shahid AA, Husnain T (2012) Transgene copy number and phenotypic variations in transgenic basmati rice. J Anim Plant Sci 22:1004–1013

    Google Scholar 

  12. Leelavathi S, Sunnichan VG, Kumria R, Vijaykanth GP, Bhatnagar RK, Reddy VS (2004) A simple and rapid Agrobacterium -mediated transformation protocol for cotton (Gossypium hirsutum L.) : Embryogenic calli as a source to generate large numbers of transgenic plants. Plant Cell Rep 22:465–470

    Article  CAS  Google Scholar 

  13. Aragao F, Vianna GR, Caravalheira SBRC, Rech EL (2005) Germ line genetic transformation in cotton (Gossypium hirsutum L.) by selection of transgenic meristematic cells with a herbicide molecule. Plant Sci 168:1227–1233

    Article  CAS  Google Scholar 

  14. Zhu SW, Gao P, Sun JS, Wang HH, Luo XM, Jiao MY, Wang ZY, Xia GX (2006) Genetic transformation of green-colored cotton. In Vitro Cell Dev Biol 42:439–444

    Article  CAS  Google Scholar 

  15. Rashid B, Saleem Z, Husnain T, Riazuddin S (2008) Transformation and inheritance of Bt genes in Gossypium hirsutum. J Plant Biol 51:248–254

    Article  CAS  Google Scholar 

  16. Ganesan M, Bhanumathi P, Ganesh KKL, Prabha A, Song PS, Jayabalan N (2009) Transgenic Indian cotton (Gossypium hirsutum) harboring rice chitinase gene (ChiII) confers resistance to two fungal pathogens. Am J Plant Biochem Biotechnol 5:6374

    Google Scholar 

  17. Miao W, Wang X, Li M, Song C, Wang Y, Hu D, Wang J (2010) Genetic transformation of cotton with a harpin-encoding gene hpaXoo confers an enhanced defense response against different pathogens through a priming mechanism. BMC Plant Biol 10:67

    Article  CAS  Google Scholar 

  18. Amudha J, Balasubramani G, Malathi VG, Monga D, Kranthi KR (2011) Cotton leaf curl virus resistance transgenics with antisense coat protein gene (AV1). Curr Sci 101:300–307

    CAS  Google Scholar 

  19. Chakrabarty PK, Kalbande B, Chavhan R, Warade J, Bajaj D, Sable S, Monga D (2011) Engineering cotton leaf curl virus resistance cotton through RNA interference approach. Mumbai: Proceedings of World Cotton Research Conference-5

    Google Scholar 

  20. Hashmi JA, Zafar Y, Arshad M, Mansoor S, Asad S (2011) Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences. Virus Genes 42:286–296

    Article  CAS  Google Scholar 

  21. Zhang B, Yang Y, Chen T, Yu W, Liu T, Li H, Chang Y (2012) Island cotton Gbve1Gene encoding a receptor-like protein confers resistance to both defoliating and non-defoliating isolates of Verticillium dahliae. PLoS One 7:e51091

    Article  CAS  Google Scholar 

  22. Liu YD, Yin ZJ, Yu JW, Li J, Wei HL, Han XL, Shen FF (2012) Improved salt tolerance and delayed leaf senescence in transgenic cotton expressing the Agrobacterium IPT gene. Biol Plant 56:237–246

    Article  CAS  Google Scholar 

  23. Yue Y, Zhang M, Zhang J, Tian X, Duan L, Li Z (2012) Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance in transgenic cotton. J Exp Bot 63:3741–3748

    Article  CAS  Google Scholar 

  24. Wang M, Zhang B, Wang Q (2013) Cotton transformation via pollen tube pathway. Methods Mol Biol 958:71–77

    Article  CAS  Google Scholar 

  25. Ali I, Amin I, Briddon RW, Mansoor S (2013) Artificial microRNA-mediated resistance against the monopartite begomovirus cotton leaf curl Burewala virus. Virol J 10:231

    Article  CAS  Google Scholar 

  26. Palle SR, Campbell LM, Pandeya D, Puckhaber L, Tollack LK, Marcel S, Rathore KS (2013) RNAi-mediated Ultra-low gossypol cotton seed trait: Performance of transgenic lines under field conditions. Plant Biotechnol J 11:296–304

    Article  CAS  Google Scholar 

  27. Vajhala CS, Sadumpati VK, Nunna HR, Puligundla SK, Vudem DR, Khareedu VR (2013) Development of transgenic cotton lines expressing Allium sativum agglutinin (ASAL) for enhanced resistance against major sap-sucking pests. PLoS One 8(9):e72542

    Article  CAS  Google Scholar 

  28. Shamim Z, Rashid B, Rahman S, Husnain T (2013) Expression of drought tolerance in transgenic cotton. Sci Asia 39:1–11

    Article  CAS  Google Scholar 

  29. Zhang B (2013) Agrobacterium-mediated transformation of cotton. Methods Mol Biol 958:31–45

    Article  CAS  Google Scholar 

  30. Khan GA, Bakhsh A, Ghazanffar M, Riazuddin S, Husnain T (2013) Development of transgenic cotton pure lines harboring a pesticidal gene (cry1Ab). Emir J Food Agric 25:434–442

    Article  Google Scholar 

  31. Bakhsh A (2014) Engineering crop plants against abiotic stress, current achievements and future prospects. Emir J Food Agric 27:24–39

    Article  Google Scholar 

  32. Mittal A, Gampala SSL, Ritchie GL, Payton P, Burke J, Rock CD (2014) Related to ABA-Insensitive3(ABI3)/Viviparous1 and AtABI5 transcription factor co-expression in cotton enhances drought stress adaptation. Plant Biotechnol J 12:578–589

    Article  CAS  Google Scholar 

  33. Bajwa KS, Shahid AA, Rao AQ, Dahab AA, Muzaffar A, Rehman HU (2014) Stable genetic transformation in cotton (Gossypium hirsutum L.) using marker genes. Adv Crop Sci 3:811–821

    Google Scholar 

  34. Song X, Kain W, Cassidy D, Wang P (2015) Resistance to Bacillus thuringiensis toxin Cry2Ab in Trichoplusia ni is conferred by a novel genetic mechanism. Appl Environ Microbiol 81:5184–5195

    Article  CAS  Google Scholar 

  35. Li B, Yang Y, Hu WR, Li XD, Cao JQ, Fan L (2015) Over-expression of GhUGP1 in upland cotton improves fibre quality and reduces fibre sugar content. Plant Breed 134:197–202

    Article  CAS  Google Scholar 

  36. Bajwa KS, Shahid AA, Rao AQ, Bashir A, Aftab A, Husnain T (2015) Stable transformation and expression of GhEXPA8 fiber expansion gene to improve fiber length and micronaire value in cotton. Front Plant Sci 6:838

    Article  Google Scholar 

  37. Sohrab SS, Kamal MA, Ilah A, Husen A, Bhattacharya PS, Rana A (2016) Development of cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene. Saudi J Biol Sci 23:358–362

    Article  CAS  Google Scholar 

  38. Murashige T, Skoog F (1962) A Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia

    S. S. Sohrab

Authors
  1. S. S. Sohrab
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Sohrab, S.S. (2019). Development of Virus Resistance Transgenic Cotton Using Cotton Leaf Curl Virus Antisense ßC1 Gene. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 1902. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8952-2_24

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8952-2_24

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8951-5

  • Online ISBN: 978-1-4939-8952-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation