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Transgenic Cotton pp 167–176Cite as

Screening of Transgenic Cotton Based on a Porous Silicon Biosensor

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1902))

Abstract

Label-free porous silicon (PSi)-based biosensor enables the detection and identification of the protein in transgenic cotton. Changes in optical response signal in the presence of the target protein can be detected by Fourier transform infrared (FTIR) spectromicroscopy when binding of the target protein with its antibody is selectively captured on the PSi biosensor. Here we describe the transgenic protein (antifreeze protein, AFP), and compare with non-transgenic plants. Significant red shifts are observed for transgenic antifreeze protein cotton lines.

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Acknowledgment

This work was supported by NSFC grant (No. 31060292) and a key grant from the Ministry of Agriculture of China (No. 2009ZX08005-022B).

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Authors and Affiliations

  1. College of Life Science and Technology, Xinjiang University, Ürümqi, China

    Liangliang Chen

  2. College of Information Science and Engineering, Xinjiang University, Ürümqi, China

    Peng Li, Xiaoyi Lv & Ji Ma

Authors
  1. Liangliang Chen
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  2. Peng Li
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  3. Xiaoyi Lv
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  4. Ji Ma
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Corresponding author

Correspondence to Ji Ma .

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Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

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Chen, L., Li, P., Lv, X., Ma, J. (2019). Screening of Transgenic Cotton Based on a Porous Silicon Biosensor. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 1902. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8952-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8952-2_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8951-5

  • Online ISBN: 978-1-4939-8952-2

  • eBook Packages: Springer Protocols

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