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Determination of Rheumatoid Factors by ELISA

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Autoantibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1901))

Abstract

IgM and IgA autoantibodies binding to IgG are called rheumatoid factors (RFs) and occur with high frequency in rheumatoid arthritis (RA) and with lower frequency in other autoimmune diseases. RFs have diagnostic and prognostic value in RA, but they also have a high potential to cause false positive reactions in other immunoassays, especially sandwich assays. For these reasons it is imperative to be able to measure RFs in serum samples from patients suspected of RA or other autoimmune diseases and in serum samples to be analyzed by sandwich immunoassay for various clinical parameters. Here, a simple ELISA for IgM and IgA RFs is described.

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Acknowledgments

The Lundbeck Foundation is thanked for a grant to Nicole H. Trier.

Author information

Authors and Affiliations

  1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Nicole Hartwig Trier & Gunnar Houen

  2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark

    Gunnar Houen

Authors
  1. Nicole Hartwig Trier
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  2. Gunnar Houen
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Corresponding author

Correspondence to Nicole Hartwig Trier .

Editor information

Editors and Affiliations

  1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

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Trier, N.H., Houen, G. (2019). Determination of Rheumatoid Factors by ELISA. In: Houen, G. (eds) Autoantibodies. Methods in Molecular Biology, vol 1901. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8949-2_23

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  • DOI: https://doi.org/10.1007/978-1-4939-8949-2_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8948-5

  • Online ISBN: 978-1-4939-8949-2

  • eBook Packages: Springer Protocols

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