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Nonspecific Binding in Immunoassays for Autoantibodies

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Autoantibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1901))

Abstract

Immunoassays are invaluable for detection and quantification of numerous analytes, including autoantibodies. However, human sera often yield high nonspecific binding in such assays, and this may result in false positive or sometimes false negative results. The causes of nonspecific binding are numerous and it correlates with inflammatory parameters. Since the results of autoantibody testing are used for diagnosis and treatment of autoimmune diseases, it is mandatory to be aware of all possible causes of nonspecific binding for each individual assay and to correct for it whenever possible. General guidelines for this are described in this chapter.

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Author information

Authors and Affiliations

  1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

  2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark

    Gunnar Houen

Authors
  1. Gunnar Houen
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Correspondence to Gunnar Houen .

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Editors and Affiliations

  1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

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Houen, G. (2019). Nonspecific Binding in Immunoassays for Autoantibodies. In: Houen, G. (eds) Autoantibodies. Methods in Molecular Biology, vol 1901. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8949-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-8949-2_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8948-5

  • Online ISBN: 978-1-4939-8949-2

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