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Antigen-Specific Detection of Autoantibodies Against Myeloperoxidase (MPO) and Proteinase 3 (PR3)

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Autoantibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1901))

Abstract

ANCA testing was introduced in many laboratories throughout the world when it was recognized that a significant subset of patients with small vessel vasculopathies presented with such antibodies. Many laboratories developed and introduced in-house testing methods for antigen-specific ANCA detection complementary to indirect immune fluorescence screening. Such in-house tests have proven their merit in diagnosing vasculitis and were important to identify critical steps in the development of antigen-specific assays with high sensitivity and specificity. In the meantime various commercial assays became available for antigen-specific ANCA testing. Because of the high diagnostic accuracy of such assays it can be anticipated that commercial, antigen-specific tests will completely replace in-house testing for MPO- and PR3-ANCA. Furthermore, such tests will replace the need for IIF in the diagnostic workup of AAV. In this light it can be foreseen that the knowledge that underlies the development of in-house ANCA testing will gradually disseminate over time. Therefore we describe the current antigen-specific ANCA ELISAs (direct and capture) with the intention to maintain the knowledge and the identification of the critical steps in the development of robust assays.

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Author information

Authors and Affiliations

  1. Central Diagnostic Laboratory, Maastricht University Medical Center, Maastricht, The Netherlands

    J. Vanderlocht, J. J. B. C. van Beers & J. Damoiseaux

  2. Department of Laboratory Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

    P. C. Limburg & C. Roozendaal

Authors
  1. J. Vanderlocht
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  2. J. J. B. C. van Beers
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  3. P. C. Limburg
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  4. J. Damoiseaux
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  5. C. Roozendaal
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Corresponding author

Correspondence to J. Damoiseaux .

Editor information

Editors and Affiliations

  1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

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Vanderlocht, J., van Beers, J.J.B.C., Limburg, P.C., Damoiseaux, J., Roozendaal, C. (2019). Antigen-Specific Detection of Autoantibodies Against Myeloperoxidase (MPO) and Proteinase 3 (PR3). In: Houen, G. (eds) Autoantibodies. Methods in Molecular Biology, vol 1901. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8949-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8949-2_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8948-5

  • Online ISBN: 978-1-4939-8949-2

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