Abstract
Isolated microspore culture (IMC) is the most efficient way to produce large numbers of doubled-haploid (DH) barley plants in a short time. Yet, while IMC is more cost-efficient and less labor-intensive than anther culture, it is technically more complex and requires more experienced personnel if it is to yield its full potential. In part, this is because of multiple and important interactions that exist between factors at its many different phases, including genotype effects as well. When every phase is fine-tuned, the protocol that is presented below yields a useful number of DHs with almost all genotypes and can allow the production of up to 300 DH plants from a single F1 plant in just a few months.
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Acknowledgments
We wish to express our gratitude to Suzanne Marchand, Martine Jean, and Isabelle Clermont from the Département de phytologie of Université Laval for their help, support, and timely suggestions.
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Esteves, P., Belzile, F.J. (2019). Isolated Microspore Culture in Barley. In: Harwood, W. (eds) Barley. Methods in Molecular Biology, vol 1900. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8944-7_5
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DOI: https://doi.org/10.1007/978-1-4939-8944-7_5
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