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Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy

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Bacteriophages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1898))

Abstract

To combat infectious diseases induced by antibiotic-resistant bacteria in human and animals, phage therapy has regained attention by the scientific community. Before phages can be widely accepted as therapeutics in the same way as antibiotics, convincing detailed applied experimental evidence must be available. The embryonated chicken egg model has been used to study the virulence of many pathogens. We describe here a procedure to test the efficacy of phage therapy to treat colibacillosis using a chicken embryo lethality assay, this being potentially applied to others bacterial infection.

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Acknowledgments

We would like to thank the personnel at PFIE (plateforme d’infectiologie expérimentale) from INRA Val de Loire for providing the SPF embryonated eggs.

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Authors and Affiliations

  1. ISP, INRA, Université de Tours, UMR 1282, 37 380, Nouzilly, France

    Angélina Trotereau & Catherine Schouler

Authors
  1. Angélina Trotereau
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  2. Catherine Schouler
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Corresponding author

Correspondence to Catherine Schouler .

Editor information

Editors and Affiliations

  1. Department of Genetics and Genome Biology, University of Leicester, Leicester, UK

    Martha R. J. Clokie

  2. Departments of Food Science, and Pathobiology, University of Guelph, Guelph, ON, Canada

    Andrew Kropinski

  3. Laboratory of Gene Technology, KU Leuven, Leuven, Belgium

    Rob Lavigne

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Trotereau, A., Schouler, C. (2019). Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy. In: Clokie, M., Kropinski, A., Lavigne, R. (eds) Bacteriophages. Methods in Molecular Biology, vol 1898. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8940-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8940-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8939-3

  • Online ISBN: 978-1-4939-8940-9

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