In Vitro Suppression of CD4+ T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cells

  • Katerina Vlachou
  • Panayotis VerginisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1899)


Myeloid-derived suppressor cells (MDSCs) are myeloid precursors of macrophages, dendritic cells, and neutrophils with a prominent role in the regulation of immune responses in cancer, infection, and autoimmunity. Herein, we describe a protocol for the isolation of murine and human MDSCs and the assessment of their ability to suppress CD4+ T-cell responses in vitro.

Key words

Myeloid-derived suppressor cells Cell sorting In vitro proliferation assay CD4+ T-cell isolation Carboxyfluorescein succinimidyl ester (CFSE) 



This work was supported by grant from the Greek General Secretariat of Research and Technology (Synergasia 09SYN-12-1074 to P.V.).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Autoimmunity and InflammationInstitute of Molecular Biology and Biotechnology, Foundation for Research and TechnologyHeraklionGreece
  2. 2.Laboratory of Cellular Immunology and Immune Tolerance, Division of Immunology and TransplantationBiomedical Research Foundation Academy of AthensAthensGreece

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