Luminex-Coupled EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Antigen-Specific T cells in Human Peripheral Blood

  • HuiJuan Lv
  • Khadir Raddassi
  • Myra A. LipesEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1899)


We describe a Luminex-coupled EliFACS assay that integrates multiplexing technology, enzyme-linked immunospot (ELISPOT), and intracellular cytokine FACS staining for the detection of multiple parameters of antigen-specific T-cell activation in human peripheral blood. Although our protocol is for measuring T-cell responses against cardiac myosin heavy chain and myelin basic protein, the major autoantigens in myocarditis and multiple sclerosis, respectively, these methods could be used for the detection of T-cell responses to other antigens, including foreign antigens.

Key words

Human T-cell assay Peripheral blood mononuclear cells Autoimmune disease ELISPOT assay 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Joslin Diabetes CenterHarvard Medical SchoolBostonUSA
  2. 2.Department of NeurologyYale School of MedicineNew HavenUSA

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