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Biobanking pp 313–323Cite as

Making a Tissue Microarray

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1897))

Abstract

Tissue microarray (TMA) is a widely used, high-throughput, cost-effective, and tissue and reagent-conserving method of performing molecular analysis. Multiple donor tissue cores are procured and transferred into a recipient TMA block for simultaneous differential and comparative molecular profiling under theoretically identical performance conditions. Described herein is a discussion of the theory behind the TMA, an overview of the concepts and principles of TMA design and construction, a brief summary of its advantages and disadvantages, and a sample protocol of TMA construction.

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Author information

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    Matthew Koo, Jill M. Squires, Daphne Ying & Jiaoti Huang

  2. Department of Pathology, Duke University School of Medicine, Durham, NC, USA

    Jiaoti Huang

Authors
  1. Matthew Koo
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  2. Jill M. Squires
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  3. Daphne Ying
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  4. Jiaoti Huang
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Corresponding author

Correspondence to Jiaoti Huang .

Editor information

Editors and Affiliations

  1. Division of Neuropathology, Department of Pathology and Laboratory Medicine, Brain Tumor Translational Resource, Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    William H. Yong

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Koo, M., Squires, J.M., Ying, D., Huang, J. (2019). Making a Tissue Microarray. In: Yong, W. (eds) Biobanking. Methods in Molecular Biology, vol 1897. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8935-5_27

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  • DOI: https://doi.org/10.1007/978-1-4939-8935-5_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8933-1

  • Online ISBN: 978-1-4939-8935-5

  • eBook Packages: Springer Protocols

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