Abstract
Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block. The paraffin block can be cut using a microtome to generate thin sections of tissue contained in paraffin to be stained or paraffin tissue ribbons suitable for nucleic acid extraction. In addition, the FFPE blocks can be stored at room temperature for years. Herein, we provide a basic knowledge, and introduce common methods of the paraffin embedding process.
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References
Van den Tweel JG, Taylor CR (2010) A brief history of pathology: preface to a forthcoming series that highlights milestones in the evolution of pathology as a discipline. Virchows Arch 457(1):3–10. https://doi.org/10.1007/s00428-010-0934-4
The National Institute of Open Schooling (2014) Histology and cytology lessons: embedding. http://www.Laboratoriumlifescience.ro. Accessed Sept 2014
Suvarna SK, Layton C, Bancroft JD (2013) Bancroft’s theory and practice of histological techniques. In: Spencer LT, Bancroft JD (eds) Tissue processing, 7th edn. Churchill Livingstone-Elsevier, China
Prophet EB, Mills B, Arrington JB et al (1992) Afip laboratory methods in histotechnology. American Registry of Pathology, Washington DC
Bancroft JD, Stevens A, Turner DR (1990) Theory and practice of histological techniques. In: Gordon KC (ed) Tissue processing, 3rd edn. Churchill Livingstone, London
Saloui M, Fiette L (2011) Histopathology procedures: from tissue sampling to histopathological evaluation. Methods Mol Biol 691:69–82. https://doi.org/10.1007/971-1-60761-849-2_4
Gray P (1958) Handbook of basic microtechnique, 2nd edn. McGraw-Hill, New York
Bass BP, Engell KB, Greytak SR et al (2014) A review of pre-analytical factors affecting molecular, protein, and morphological analysis of formalin-fixed, paraffin-embedded (FFPE) tissue. Arch Pathol Lab Med 138(11):1520–1530. https://doi.org/10.5858/arpa.2013-0691-RA
Hewitt SM, Lewis FA, Cao Y et al (2008) Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin embedded tissue. Arch Pathol Lab Med 132(12):1929–1933. https://doi.org/10.1043/1543-2162-132.121929
Rolls G (2008) 101 steps to better histology. http://www.leicabiosystems.com. Accessed 16 June 2008
Acknowledgments
We are grateful to Nima Sotoudeh, a graphic designer and student of animation at the University of Technology Sydney (UTS), Australia, for his artful illustrations used in this chapter.
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Sadeghipour, A., Babaheidarian, P. (2019). Making Formalin-Fixed, Paraffin Embedded Blocks. In: Yong, W. (eds) Biobanking. Methods in Molecular Biology, vol 1897. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8935-5_22
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DOI: https://doi.org/10.1007/978-1-4939-8935-5_22
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