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IMR90 ER:RAS: A Cell Model of Oncogene-Induced Senescence

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Cellular Senescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1896))

Abstract

Oncogene-induced senescence (OIS) is a cellular response that limits the replication of cells expressing oncogenes. As a result, OIS is a potent tumor suppressor mechanism limiting cancer progression. Here we describe IMR90 ER:RAS, a widely used model to study OIS in cell culture. This model takes advantage of IMR90 human primary fibroblast infected with a 4-hydroxy-tamoxifen (4-OHT) inducible ER:RAS construct. RAS activation upon 4-OHT treatment results in a coordinated induction of senescence, recapitulating different aspects of the phenotype such as the growth arrest and the establishment of a senescence-associated secretory phenotype (SASP).

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Acknowledgments

This work was supported by core funding from the Medical Research Council, London Institute of Medical Sciences. A.J.I. is supported by a National Institute for Health Research (NIHR) Clinical Lectureship, and acknowledges support from the NIHR and Imperial Biomedical Research Centre (BRC).

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Authors and Affiliations

  1. MRC London Institute of Medical Sciences (LMS), London, UK

    Andrew J. Innes & Jesús Gil

  2. Faculty of Medicine, Institute of Clinical Sciences (ICS), Imperial College London, London, UK

    Andrew J. Innes & Jesús Gil

  3. Faculty of Medicine, Centre for Haematology, Imperial College London, London, UK

    Andrew J. Innes

Authors
  1. Andrew J. Innes
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  2. Jesús Gil
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Correspondence to Jesús Gil .

Editor information

Editors and Affiliations

  1. ERIBA, University Medical Center Groningen, Groningen, Groningen, The Netherlands

    Marco Demaria

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Innes, A.J., Gil, J. (2019). IMR90 ER:RAS: A Cell Model of Oncogene-Induced Senescence. In: Demaria, M. (eds) Cellular Senescence. Methods in Molecular Biology, vol 1896. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8931-7_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8931-7_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8930-0

  • Online ISBN: 978-1-4939-8931-7

  • eBook Packages: Springer Protocols

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