Abstract
Senescent cells display altered metabolic signatures that can either be causal for aspects the senescent phenotype, or act as biomarkers for senescence. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography–mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.
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References
Quijano C et al (2012) Oncogene-induced senescence results in marked metabolic and bioenergetic alterations. Cell Cycle 11(7):1383–1392
Wiley CD, Campisi J (2016) From ancient pathways to aging cells-connecting metabolism and cellular senescence. Cell Metab 23(6):1013–1021
Zwerschke W et al (2003) Metabolic analysis of senescent human fibroblasts reveals a role for AMP in cellular senescence. Biochem J 376(Pt 2):403–411
Bajad SU et al (2006) Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry. J Chromatogr A 1125(1):76–88
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Wiley, C.D., Davis, S., Ramanathan, A. (2019). Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry. In: Demaria, M. (eds) Cellular Senescence. Methods in Molecular Biology, vol 1896. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8931-7_13
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DOI: https://doi.org/10.1007/978-1-4939-8931-7_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8930-0
Online ISBN: 978-1-4939-8931-7
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