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A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in Fluids

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Cellular Senescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1896))

Abstract

Lipofuscin accumulation is a hallmark of senescence. This nondegradable material aggregates in the cytoplasm of stressed or damaged cells due to metabolic imbalance associated with aging and age-related diseases. Indications of a soluble state of lipofuscin have also been provided, rendering the perspective of monitoring such processes via lipofuscin quantification in liquids intriguing. Therefore, the development of an accurate and reliable method is of paramount importance. Currently available assays are characterized by inherent pitfalls which demote their credibility. We herein describe a simple, highly specific and sensitive protocol for measuring lipofuscin levels in any type of liquid. The current method represents an evolution of a previously described assay, developed for in vitro and in vivo senescent cell recognition that exploits a newly synthesized Sudan Black-B analog (GL13). Analysis of human clinical samples with the modified protocol provided strong evidence of its usefulness for the exposure and surveillance of age-related conditions.

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Change history

  • 07 February 2019

    In Chapter 12, figure 1 was published with truncated texts within. This figure has now been replaced with a revised figure with the updated text.

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Acknowledgments

We would like to thank Dr. Alexandros Papalampros and Dr. Dimitrios Papadopoulos for providing material for this investigation. This work was financially supported by the “SYNTRAIN” ITN Horizon 2020 Grant No 722729, the NKUA SARG grants 70/3/12128, 70/3/8916, 70/3/1135 and the Welfare Foundation for Social & Cultural Sciences (KIKPE) Greece.

Conflict of interest:

The authors wish to declare no conflict of interest.

Patent pending: UK Patent Application No GB1803531.1.

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Author notes

  1. Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, and Iordanis Mourouzis contributed equally to this work.

Authors and Affiliations

  1. Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece

    Sophia V. Rizou, Konstantinos Evangelou, Sophia Havaki, Panagiotis V. S. Vasileiou, Aggelos Margetis, Athanassios Kotsinas, Nikolaos G. Kastrinakis & Vassilis G. Gorgoulis

  2. Department of Anatomy-Histology-Embryology, Medical School, University of Ioannina, Ioannina, Greece

    Konstantinos Evangelou

  3. Division of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece

    Vassilios Myrianthopoulos & Emmanuel Mikros

  4. PharmaInformatics Unit, Athena Research Center, Athens, Greece

    Vassilios Myrianthopoulos & Emmanuel Mikros

  5. Department of Pharmacology, Medical School, National and Kapodistrian University of Athens, Athens, Greece

    Iordanis Mourouzis & Constantinos Pantos

  6. Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, NY, USA

    Aikaterini Athanasiou

  7. First Department of Propaedeutic Internal Medicine and Rheumatology Unit, Medical School, National and Kapodistrian University of Athens, Athens, Greece

    Petros Sfikakis

  8. Faculty Institute for Cancer Sciences, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK

    Paul Townsend & Vassilis G. Gorgoulis

  9. Biomedical Research Foundation, Academy of Athens, Athens, Greece

    Vassilis G. Gorgoulis

  10. Center for New Biotechnologies and Precision Medicine, Medical School, National and Kapodistrian University of Athens, Athens, Greece

    Vassilis G. Gorgoulis

Authors
  1. Sophia V. Rizou
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  2. Konstantinos Evangelou
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  3. Vassilios Myrianthopoulos
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  4. Iordanis Mourouzis
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  5. Sophia Havaki
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  6. Aikaterini Athanasiou
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  7. Panagiotis V. S. Vasileiou
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  8. Aggelos Margetis
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  9. Athanassios Kotsinas
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  10. Nikolaos G. Kastrinakis
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  11. Petros Sfikakis
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  12. Paul Townsend
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  13. Emmanuel Mikros
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  14. Constantinos Pantos
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  15. Vassilis G. Gorgoulis
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Corresponding author

Correspondence to Vassilis G. Gorgoulis .

Editor information

Editors and Affiliations

  1. ERIBA, University Medical Center Groningen, Groningen, Groningen, The Netherlands

    Marco Demaria

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Rizou, S.V. et al. (2019). A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in Fluids. In: Demaria, M. (eds) Cellular Senescence. Methods in Molecular Biology, vol 1896. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8931-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8931-7_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8930-0

  • Online ISBN: 978-1-4939-8931-7

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