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Measurement of Nanoparticle-Induced Mitochondrial Membrane Potential Alterations

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Nanotoxicity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1894))

Abstract

Mitochondria hold a critical role in cell metabolism and homeostasis. Mitochondrial injury plays central part in deciding cell fate especially in programmed cell death pathways. Various nanomaterials lead to different cell death modalities by inducing mitochondrial injury. Mitochondrial injury is manifested as multiple biochemical events ranging from altered energy production, mitochondrial outer membrane permeability, release of pro-apoptotic BCl-2 family proteins, loss of mitochondrial inner membrane potential, mitochondrial swelling, and disruption of mitochondrial structure leading to eventual lysis of mitochondria. Mitochondrial membrane permeability (loss of mitochondrial membrane potential) is a critical event in deciding cell fate. This chapter presents an overview of nanomaterial-induced loss of mitochondrial membrane potential and discusses potential nano-specific artifacts in these assays. Finally, a detailed methodology to accurately quantify and validate the loss of mitochondrial membrane potential after nanomaterial exposures is presented.

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, Robert C. Byrd Health Science Center, School of Medicine,, West Virginia University, Morgantown, WV, USA

    Salik Hussain

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  1. Salik Hussain
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Correspondence to Salik Hussain .

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Editors and Affiliations

  1. Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA

    Qunwei Zhang

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Hussain, S. (2019). Measurement of Nanoparticle-Induced Mitochondrial Membrane Potential Alterations. In: Zhang, Q. (eds) Nanotoxicity. Methods in Molecular Biology, vol 1894. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8916-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8916-4_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8915-7

  • Online ISBN: 978-1-4939-8916-4

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