Abstract
Stem cells are undifferentiated biological cells that can differentiate into all lineages under defined control condition. Stem cell neuronal differentiation can faithfully recapitulate stages of neural development and generate neuronal progenitors, mature neurons, and glial cells. Stem cell technology will largely allow for the replacement of animal studies and reduce costs, and will provide a new paradigm for in toxic genomics, bioinformatics, systems biology, and epigenetics studies. Here, we describe a nonadherent neuronal differentiation methodology developed in our laboratory, which can rapidly derive neurons and astrocytes from human embryonic stem cells (hESCs) and induced pluripotent stem cell (hiPSC) and use of this platform for nanoparticle neurotoxicity study.
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References
Abbondanzo SJ, Gadi I, Stewart CL (1993) Derivation of embryonic stem cells. Methods Enzymol 225:803–823
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126(4):663–676
Zhang SC, Wernig M, Duncan ID, Brüstle O, Thomson JA (2001) In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol 19(12):1129–1133
Hattori N (2014) Cerebral organoids model human brain development and microcephaly. Nature 501(7467):373–379
van dan Ameele J, Tiberi L, Vanderhaeghen P, Espuny-Camcho I (2014) Thinking out of the dish: what to learn about cortical development using pluripotent stem cells. Trends Neurosci 37(6):334–342
Shi Y, Kirwan P, Livesey FJ (2012) Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat Protoc 7(10):1836–1846
Begum AN, Guoynes C, Cho J, Hao J, Lutfy K, Hong Y (2015) Rapid generation of sub-type, region-specific neurons and neural networks from human pluripotent stem cell-derived neurospheres. Stem Cell Res 15(3):731–741
Begum AN, Aguilar JS, Elias L, Hong Y (2016) Silver nanoparticles exhibit coating and dose-dependent neurotoxicity in glutamatergic neurons derived from human embryonic stem cells. Neurotoxicology 57:45–53
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Hong, Y., Chan, N., Begum, A.N. (2019). Deriving Neural Cells from Pluripotent Stem Cells for Nanotoxicity Testing. In: Zhang, Q. (eds) Nanotoxicity. Methods in Molecular Biology, vol 1894. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8916-4_4
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DOI: https://doi.org/10.1007/978-1-4939-8916-4_4
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8916-4
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