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Nanotoxicity Assessment Using Embryonic Zebrafish

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Nanotoxicity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1894))

Abstract

The emergence of nanomaterials in industrial processing and consumer products has generated an increased presence of nano-enabled products in the environment and now pose an increased risk of exposure to living organisms. However, assessing the risks of nanomaterials is a challenging task because of a large variety and great variability in their properties. Here, we describe a methodology for assessing toxicity and evaluate potential risks posed by nanomaterials using zebrafish embryos as a model organism. Zebrafish are a well-established organism that has a number of advantages over other biological models. These include optical transparency, similar structure and arrangement of organs, and conserved genetic pathways compared to other vertebrates. Their rapid development and high numbers of embryos enables high throughput screening to study toxicity of a large number of nanomaterials. The method described in this chapter can be used as a universal screening approach to assess toxicity of any type of nanomaterials, determine both lethal and sublethal effects, measure LD50 doses, evaluate morphological and organ defects, cell apoptosis, and production of reactive species.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Eduard Dumitrescu & Silvana Andreescu

  2. Department of Biology, Clarkson University, Potsdam, NY, USA

    Kenneth Wallace

Authors
  1. Eduard Dumitrescu
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  2. Kenneth Wallace
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  3. Silvana Andreescu
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Corresponding author

Correspondence to Silvana Andreescu .

Editor information

Editors and Affiliations

  1. Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA

    Qunwei Zhang

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Dumitrescu, E., Wallace, K., Andreescu, S. (2019). Nanotoxicity Assessment Using Embryonic Zebrafish. In: Zhang, Q. (eds) Nanotoxicity. Methods in Molecular Biology, vol 1894. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8916-4_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8916-4_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8915-7

  • Online ISBN: 978-1-4939-8916-4

  • eBook Packages: Springer Protocols

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