Abstract
Rice is one of the staple foods which serves as the major source of carbohydrate in the human diet. A typical milled rice grain is mainly composed of starch of up to 80–90%, with an average of 6–8% proteins and some trace amounts of dietary fiber. Although cooked white rice can elicit variable glycemic response, a portion of rice starch may evade digestion in the human small intestine. The digested portion of rice can be estimated and characterized in vitro based on starch digestion extent and rate (kinetics). The indigestible portion of starch can also be quantified. This chapter will present micro-scale methods to quantify rice starch digestion rate and extent based on the sugar fractions released after treating the samples with digestive enzymes.
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References
Butardo VM Jr, Sreenivasulu N (2016) Tailoring grain storage reserves for a healthier rice diet and its comparative status with other cereals. Int Rev Cell Mol Biol 323:31–70
FAO (1998) Dietary carbohydrate composition. Food and Agriculture Organization of the United Nations, Rome
Sparti A, Milon H, Di Vetta V, Schneiter P, Tappy L, Jequier E, Schutz Y (2000) Effect of diets high or low in unavailable and slowly digestible carbohydrates on the pattern of 24-h substrate oxidation and feelings of hunger in humans. Am J Clinical Nut 72(6):1461–1468
Englyst HN, Cummings JH (1987) Digestion of polysaccharides of potato in the small-intestine of man. Am J Clin Nutr 45(2):423–431
Englyst HN, Macfarlane GT (1986) Breakdown of resistant and readily digestible starch by human gut bacteria. J Sci Food Agric 37(7):699–706
Ranganathan S, Champ M, Pechard C, Blanchard P, Nguyen M, Colonna P, Krempf M (1994) Comparative-study of the acute effects of resistant starch and dietary-fibers on metabolic indexes in men. Am J Clin Nutr 59(4):879–883
Topping DL, Clifton PM (2001) Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiol Rev 81(3):1031–1064
Behall KM, Scholfield DJ, Hallfrisch JG, Liljeberg-Elmstahl HGM (2006) Consumption of both resistant starch and beta-glucan improves postprandial plasma glucose and insulin in women. Diabetes Care 29(5):976–981
World Health Organization (2002) The World Health Report: Reducing risks, promoting healthy life
Dhital S, Dabit L, Zhang B, Flanagan B, Shrestha AK (2015) In vitro digestibility and physicochemical properties of milled rice. Food Chem 172:757–765
Butardo VM Jr, Anacleto R, Parween S, Samson I, de Guzman K, Alhambra CM, Misra G, Sreenivasulu N (2017) Systems genetics identifies a novel regulatory domain of amylose synthesis. Plant Physiol 173(1):887–906
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Alhambra, C.M., Dhital, S., Sreenivasulu, N., Butardo, V.M. (2019). Quantifying Grain Digestibility of Starch Fractions in Milled Rice. In: Sreenivasulu, N. (eds) Rice Grain Quality. Methods in Molecular Biology, vol 1892. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8914-0_13
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DOI: https://doi.org/10.1007/978-1-4939-8914-0_13
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