Abstract
Examining the subcellular localization of Hippo pathway components has helped elucidate the molecular mechanisms that regulate the pathway. Here we describe methods for performing live imaging of fluorescently tagged Hippo pathway components in Drosophila wing imaginal discs.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Oh H, Irvine KD (2008) In vivo regulation of Yorkie phosphorylation and localization. Development 135:1081–1088. https://doi.org/10.1242/dev.015255
Su T, Ludwig MZ, Xu J, Fehon RG (2017) Kibra and Merlin activate the Hippo pathway spatially distinct from and independent of expanded. Dev Cell 40:478–490.e3. https://doi.org/10.1016/j.devcel.2017.02.004
Rauskolb C, Sun S, Sun G et al (2014) Cytoskeletal tension inhibits Hippo signaling through an Ajuba-Warts complex. Cell 158:143–156. https://doi.org/10.1016/j.cell.2014.05.035
Boggiano JC, Fehon RG (2012) Growth control by committee: intercellular junctions, cell polarity, and the cytoskeleton regulate Hippo signaling. Dev Cell 22:695–702. https://doi.org/10.1016/j.devcel.2012.03.013
Sun S, Reddy BVVG, Irvine KD (2015) Localization of Hippo signalling complexes and Warts activation in vivo. Nat Commun 6:8402. https://doi.org/10.1038/ncomms9402
Chung H-LL, Augustine GJJ, Choi K-WW (2016) Drosophila Schip1 links expanded and Tao-1 to regulate Hippo signaling. Dev Cell 36:511–524. https://doi.org/10.1016/j.devcel.2016.02.004
Dupont S, Morsut L, Aragona M et al (2011) Role of YAP/TAZ in mechanotransduction. Nature 474:179–183. https://doi.org/10.1038/nature10137
Aragona M, Panciera T, Manfrin A et al (2013) A mechanical checkpoint controls multicellular growth through YAP/TAZ regulation by actin-processing factors. Cell 154(5):1047–1059. https://doi.org/10.1016/j.cell.2013.07.042
Acknowledgments
T.S. and J.X. were recipients of the Children’s Tumor Foundation Young Investigator Award (2012-01-033, 2013-01-020, and 2014-01-020, respectively), and S.T. was supported by NIH training grant T32 GM007183. This work was supported by a grant from the NIH to R.G.F. (NS034783).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Xu, J., Su, T., Tokamov, S.A., Fehon, R.G. (2019). Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_4
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8910-2_4
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8909-6
Online ISBN: 978-1-4939-8910-2
eBook Packages: Springer Protocols