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Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs

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The Hippo Pathway

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

Abstract

Examining the subcellular localization of Hippo pathway components has helped elucidate the molecular mechanisms that regulate the pathway. Here we describe methods for performing live imaging of fluorescently tagged Hippo pathway components in Drosophila wing imaginal discs.

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References

  1. Oh H, Irvine KD (2008) In vivo regulation of Yorkie phosphorylation and localization. Development 135:1081–1088. https://doi.org/10.1242/dev.015255

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Su T, Ludwig MZ, Xu J, Fehon RG (2017) Kibra and Merlin activate the Hippo pathway spatially distinct from and independent of expanded. Dev Cell 40:478–490.e3. https://doi.org/10.1016/j.devcel.2017.02.004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Rauskolb C, Sun S, Sun G et al (2014) Cytoskeletal tension inhibits Hippo signaling through an Ajuba-Warts complex. Cell 158:143–156. https://doi.org/10.1016/j.cell.2014.05.035

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Boggiano JC, Fehon RG (2012) Growth control by committee: intercellular junctions, cell polarity, and the cytoskeleton regulate Hippo signaling. Dev Cell 22:695–702. https://doi.org/10.1016/j.devcel.2012.03.013

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Sun S, Reddy BVVG, Irvine KD (2015) Localization of Hippo signalling complexes and Warts activation in vivo. Nat Commun 6:8402. https://doi.org/10.1038/ncomms9402

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Chung H-LL, Augustine GJJ, Choi K-WW (2016) Drosophila Schip1 links expanded and Tao-1 to regulate Hippo signaling. Dev Cell 36:511–524. https://doi.org/10.1016/j.devcel.2016.02.004

    Article  CAS  PubMed  Google Scholar 

  7. Dupont S, Morsut L, Aragona M et al (2011) Role of YAP/TAZ in mechanotransduction. Nature 474:179–183. https://doi.org/10.1038/nature10137

    Article  CAS  PubMed  Google Scholar 

  8. Aragona M, Panciera T, Manfrin A et al (2013) A mechanical checkpoint controls multicellular growth through YAP/TAZ regulation by actin-processing factors. Cell 154(5):1047–1059. https://doi.org/10.1016/j.cell.2013.07.042

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

T.S. and J.X. were recipients of the Children’s Tumor Foundation Young Investigator Award (2012-01-033, 2013-01-020, and 2014-01-020, respectively), and S.T. was supported by NIH training grant T32 GM007183. This work was supported by a grant from the NIH to R.G.F. (NS034783).

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Correspondence to Richard G. Fehon .

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Xu, J., Su, T., Tokamov, S.A., Fehon, R.G. (2019). Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8909-6

  • Online ISBN: 978-1-4939-8910-2

  • eBook Packages: Springer Protocols

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