Skip to main content
SpringerLink
Log in
Book cover

The Hippo Pathway pp 43–51Cite as

Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

Abstract

Drosophila melanogaster has been widely used in the study of developmental growth control and has been instrumental in the discovery and delineation of many signalling pathways that contribute to this growth, in particular the Hippo pathway. Quantitative analysis of adult tissue size has remained a vital tool in the study of tissue growth. This chapter will describe how to dissect, image, and quantify two tissues commonly used to measure growth, the Drosophila wing and eye.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. St Johnston D (2002) The art and design of genetic screens: Drosophila melanogaster. Nat Rev Genet 3(3):176–188. https://doi.org/10.1038/nrg751

    Article  CAS  PubMed  Google Scholar 

  2. Hariharan IK, Bilder D (2006) Regulation of imaginal disc growth by tumor-suppressor genes in Drosophila. Annu Rev Genet 40:335–361. https://doi.org/10.1146/annurev.genet.39.073003.100738

    Article  CAS  PubMed  Google Scholar 

  3. Justice R, Zilian O, Woods D, Noll M, Bryant P (1995) The Drosophila tumor suppressor gene warts encodes a homolog o-f human myotonic dystrophy kinase and is required for the control of cell shape and proliferation. Genes Dev 9:534–546

    Article  CAS  PubMed  Google Scholar 

  4. Harvey K, Tapon N (2007) The Salvador-Warts-Hippo pathway - an emerging tumour-suppressor network. Nat Rev Cancer 7(3):182–191. https://doi.org/10.1038/nrc2070

    Article  CAS  PubMed  Google Scholar 

  5. Pan D (2010) The hippo signaling pathway in development and cancer. Dev Cell 19(4):491–505. https://doi.org/10.1016/j.devcel.2010.09.011

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Bate M, Arias AM (1991) The embryonic origin of imaginal discs in Drosophila. Development 112(3):755–761

    CAS  PubMed  Google Scholar 

  7. Fristrom DK, Fristrom JW (1993) The metamorphic development of the adult epidermis. The Development of Drosophila melanogaster. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  8. de la Loza MC D, Thompson BJ (2017) Forces shaping the Drosophila wing. Mech Dev 144(Pt A):23–32. https://doi.org/10.1016/j.mod.2016.10.003

    Article  CAS  Google Scholar 

  9. Cagan R (2009) Principles of Drosophila eye differentiation. Curr Top Dev Biol 89:115–135. https://doi.org/10.1016/s0070-2153(09)89005-4

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Hariharan IK (2015) Organ size control: lessons from Drosophila. Dev Cell 34(3):255–265. https://doi.org/10.1016/j.devcel.2015.07.012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Brand AH, Perrimon N (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development:118

    Google Scholar 

  12. Kulaberoglu Y, Lin K, Holder M, Gai Z, Gomez M, Assefa Shifa B, Mavis M, Hoa L, Sharif AAD, Lujan C, Smith ESJ, Bjedov I, Tapon N, Wu G, Hergovich A (2017) Stable MOB1 interaction with Hippo/MST is not essential for development and tissue growth control. Nat Commun 8:695. https://doi.org/10.1038/s41467-017-00795-y

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Stern DL, Sucena E (2012) Preparation and mounting of adult Drosophila structures in Canada balsam. Cold Spring Harb Protoc 2012(3):373–375. https://doi.org/10.1101/pdb.prot067389

    Article  PubMed  Google Scholar 

  14. Ashburner M, Golic KG, Scott Hawley R (1989) Drosophila: a laboratory handbook. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

Download references

Acknowledgments

We thank Maxine Holder and Nic Tapon for comments on the manuscript. This work was supported by funding from Cancer Research UK (C16420/A18066) and from The Academy of Medical Sciences/Wellcome Trust Springboard Award (SBF001/1018).

Author information

Authors and Affiliations

  1. Centre for Tumour Biology, Barts Cancer Institute, Queen Mary University of London, London, UK

    Alexander D. Fulford & Paulo S. Ribeiro

Authors
  1. Alexander D. Fulford
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paulo S. Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Paulo S. Ribeiro .

Editor information

Editors and Affiliations

  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Fulford, A.D., Ribeiro, P.S. (2019). Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8909-6

  • Online ISBN: 978-1-4939-8910-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation