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Lactic Acid Bacteria pp 85–94Cite as

Preparation of Exopolysaccharide Synthesized by Lactic Acid Bacteria

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1887))

Abstract

Polysaccharides produced by lactic acid bacteria are divided into two classes based on their biosynthesis and structure. Nevertheless, the procedures for isolation, purification, and chemical analysis of these polymers are very similar. This chapter introduces the techniques that are used to prepare exopolysaccharides from lactic acid bacteria; both large- and small-scale preparation methods are described. The chapter consists of three sections, covering precipitation (using ethanol or trichloroacetic acid), ion-exchange chromatography, and ultrafiltration. Technical advisory notes on the individual steps are also provided.

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Authors and Affiliations

  1. Department of Life and Environmental Science, Faculty of Engineering, Hachinohe Institute of Technology, Hachinohe, Aomori, Japan

    Junko Nishimura

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  1. Junko Nishimura
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Correspondence to Junko Nishimura .

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Editors and Affiliations

  1. Department of Food Management, Miyagi University, Sendai, Miyagi, Japan

    Makoto Kanauchi

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Nishimura, J. (2019). Preparation of Exopolysaccharide Synthesized by Lactic Acid Bacteria. In: Kanauchi, M. (eds) Lactic Acid Bacteria. Methods in Molecular Biology, vol 1887. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8907-2_8

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  • DOI: https://doi.org/10.1007/978-1-4939-8907-2_8

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8906-5

  • Online ISBN: 978-1-4939-8907-2

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