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Adhesion of Lactobacillus to Intestinal Mucin

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Lactic Acid Bacteria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1887))

Abstract

Adhesion to intestinal mucin is one of the main in vitro tests for the study of probiotic strains. Mucins immobilized on microtiter plates and glass slides are easy and excellent methods used for the quantitative analysis of Lactobacillus adhesion. However, to maintain the performance of the quantitative analysis, these methods must be chosen appropriately according to the nature and characteristics of the strain, such as tolerance to surfactant and the ability to self-aggregate. Here we describe two methodologies used to evaluate adhesion of Lactobacillus to mucin, in addition to the isolation and purification of mucins from porcine colonic tissues.

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Authors and Affiliations

  1. Department of Microbiology, School of Pharmacy, Kitasato University, Tokyo, Japan

    Keita Nishiyama

  2. Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan

    Takao Mukai

Authors
  1. Keita Nishiyama
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  2. Takao Mukai
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Corresponding author

Correspondence to Keita Nishiyama .

Editor information

Editors and Affiliations

  1. Department of Food Management, Miyagi University, Sendai, Miyagi, Japan

    Makoto Kanauchi

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Nishiyama, K., Mukai, T. (2019). Adhesion of Lactobacillus to Intestinal Mucin. In: Kanauchi, M. (eds) Lactic Acid Bacteria. Methods in Molecular Biology, vol 1887. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8907-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8907-2_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8906-5

  • Online ISBN: 978-1-4939-8907-2

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