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Generation and Identification of Genetically Modified Mice for BMP Receptors

  • Jingwen Yang
  • Yuji Mishina
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1891)

Abstract

BMP signaling is critical in embryogenesis and in the development of numerous tissues. Many genetically modified (knockout and transgenic) mice have been established to study BMP function in development and disease. Mice with altered BMP receptor genes (including global knockout, conditional knockout, and conditional constitutively active transgenic mouse lines) have been particularly informative. In this chapter, we describe how the genetically modified mice were generated and introduce genotyping methods. These methods include regular PCR and genomic real-time PCR using specific primers based on different constructs in different mice strains.

Key words

Transgenic BMP receptors Regular PCR Genomic real-time PCR Primers 

Notes

Acknowledgments

We thank Drs. Ce Shi and Taocong Jin for designing probe sets for quantitative PCR. We are grateful to Dr. Kaitrin Kramer for critical reading of this manuscript. This work was supported by the National Institutes of Health (R01DE020843 to Y.M.), International FOP Association (Y.M.), the grant-in-aid from the National Natural Science Foundation of China (31500788 to J.Y.), and the Fundamental Research Fund for the Central Universities of China (410500114 to J.Y.).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biologic and Materials SciencesSchool of Dentistry, University of MichiganAnn ArborUSA
  2. 2.State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM)School and Hospital of Stomatology, Wuhan UniversityWuhanChina

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