Abstract
FOXO proteins represent a subfamily of transcription factors that belong to the forkhead family. The study of FOXO target genes can be performed using Real-Time PCR (RT-PCR). The RT-PCR is a sensitive method that allows the detection and quantification of minute amounts of nucleic acids. In RT-PCR the accumulation of the amplicon is detected and measured as the reaction progresses. Here, we describe the application of RT-PCR technique to monitor the transcriptional activity of FOXO transcription factors.
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Henriques, V., Machado, S., Link, W., Ferreira, B.I. (2019). Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes. In: Link, W. (eds) FOXO Transcription Factors. Methods in Molecular Biology, vol 1890. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8900-3_9
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DOI: https://doi.org/10.1007/978-1-4939-8900-3_9
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