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Myogenesis pp 111–125Cite as

Adipogenesis from Bovine Precursors

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1889))

Abstract

Cultured meat, or tissue engineered meat, is a promising alternative to conventional meat production. In order to realistically mimic the multiple tissue types found in beef, food-compatible methods for bovine fat tissue engineering must be developed. We present a protocol for the isolation of adipose tissue-derived preadipocytes and subsequent adipogenic differentiation through free fatty acid stimulation. Differentiating preadipocytes can be either grown in 2D culture conditions or seeded in 3D alginate scaffolds. Differentiation is visually confirmed through lipophilic staining.

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Acknowledgments

This work was supported by the Brin-Wojcicki Foundation, the Fulbright U.S. Student Program, and the Netherland-America Foundation.

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Authors and Affiliations

  1. Department of Physiology, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands

    Frea Mehta, Ruud Theunissen & Mark J. Post

Authors
  1. Frea Mehta
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  2. Ruud Theunissen
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  3. Mark J. Post
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Corresponding author

Correspondence to Mark J. Post .

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Editors and Affiliations

  1. Nofima AS, Ås, Norway

    Sissel Beate Rønning

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Mehta, F., Theunissen, R., Post, M.J. (2019). Adipogenesis from Bovine Precursors. In: Rønning, S. (eds) Myogenesis. Methods in Molecular Biology, vol 1889. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8897-6_8

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  • DOI: https://doi.org/10.1007/978-1-4939-8897-6_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8896-9

  • Online ISBN: 978-1-4939-8897-6

  • eBook Packages: Springer Protocols

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