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Myogenesis pp 95-110 | Cite as

Lentivirus-Mediated RNAi in Skeletal Myogenesis

  • Dongwook Kim
  • Adriana Reyes-Ordoñez
  • Jie ChenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1889)

Abstract

RNA interference (RNAi) has greatly facilitated investigation of gene functions in vitro as well as in vivo. Recombinant lentivirus is widely used to deliver small hairpin RNA (shRNA) because of its high transduction capacity into diverse cell types and tissues. Here, we describe methods of lentivirus-mediated delivery of shRNA for the study of skeletal muscle cell differentiation in vitro and injury-induced muscle regeneration in mice.

Key words

RNAi shRNA Lentivirus Gene knockdown Skeletal myogenesis Muscle regeneration 

Notes

Acknowledgment

We thank members of the Chen laboratory for developing and refining the protocols described here. This work was supported by NIH grant R01 AR048914.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Dongwook Kim
    • 1
  • Adriana Reyes-Ordoñez
    • 1
  • Jie Chen
    • 1
    Email author
  1. 1.Department of Cell and Developmental BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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